铜色牛肝菌多糖的结构表征及抗氧化活性研究

Extraction and Antioxidant Activity of Polysaccharides from Boletus Aereus

通过加热提取铜色牛肝菌(Boletus aereus)多糖,采用30%双氧水进行脱色、Sevag法除蛋白,通过FT-IR分析证明BA-2多糖存在β构型的吡喃糖。柱前衍生化HPLC证明多糖含有葡萄糖、甘露糖和半乳糖三种单糖。尺寸排除色谱结合多角度激光光散射和示差检测器分析多糖的M_(w)为7.760×10^(5)Da和M_(n)为2.671×10^(4)Da,其多分散系数M_(w)/M_(n)为29.049,其多分散系数值很大,表明BA-2多糖的相对分子质量分布很宽。在BA-2质量浓度为10 mg/mL时,对ABTS自由基和DPPH自由基的清除能力分别为50.07%和32.2%,表明BA-2对ABTS自由基清除效果更好。这些结果为更好地开发和利用这一资源提供了理论依据,实现更好的经济效益和社会效益。因此,有必要开展对铜色牛肝菌多糖的抗氧化研究,以充分发挥其潜在的药用价值。

Extraction of polysaccharides from Boletus aereus using the heating method,followed by decolorization with 30%hydrogen peroxide and protein removal with Sevag method.FT-IR analysis confirmed the presence ofβ-type pyranose in BA-2 polysaccharides.Pre-derivatization HPLC showed the presence of glucose,mannose,and galactose in the polysaccharides.Size-Exclusion Chromatography combined with multi-angle laser light scattering and differential detectors analyzed the polysaccharide′s M_(w)to be 7.760×10^(5)Da and M_(n)to be 2.671×104 Da,with a polydispersity index of 29.049,indicating that the molecular weight distribution of BA-2 polysaccharide is broad.At BA-2 of 10 mg/mL,the clearance ability of ABTS and DPPH free radicals was 50.07%and 32.2%,respectively,indicating a better clearance effect of ABTS free radicals by BA-2.These results provide a theoretical basis for the better development and utilization of this resource,achieving better economic and social benefits.Therefore,it is necessary to conduct antioxidant research on the polysaccharides of Boletus aereus to fully tap into its potential medicinal value.