基于转录组深度测序研究银杏中萜类合成酶的功能

Study on the Function of Terpenoid Synthetase in Ginkgo Biloba L.Based on Transcriptome Deep Sequencing

目的:利用深度转录组测序,获得了8个银杏萜类合成酶基因。方法:通过生物信息学方法分析,推测了8个银杏萜类合成酶基因的功能。结果:系统发育树表明GbTPS6基因属于TPS-f家族,其功能更倾向于被子植物的功能。GbTPS1,GbTPS2和GbTPS5属于TPS-d3亚家族,该家族是裸子植物倍半萜合成酶的亚家族。GbTPS4属于TPS-d2亚家族,属于二萜合成酶的亚家族。结论:基于银杏深度转录组测序,通过生物化学、生物信息学以及合成生物学等方法,对银杏中的萜类合成酶进行基因功能分析,为未来更深入研究银杏中的萜类合成酶提供研究思路和方法。

Objective:Eight Ginkgo biloba terpenoid synthase genes were obtained using deep transcriptome sequencing.Methods:Through bioinformatics analysis,the functions of eight Ginkgo biloba terpenoid synthase genes were speculated.Results:The phylogenetic tree indicates that the GbTPS6 gene belongs to the TPS-f family and its function is more inclined towards that of angiosperms.GbTPS1,GbTPS2,and GbTPS5 belong to the TPS-d3 subfamily,which is a subfamily of sesquiterpene synthase in gymnosperms.GbTPS4 belongs to the TPS-d2 subfamily and belongs to the subfamily of diterpene synthase.Conclusion:Based on deep transcriptome sequencing of Ginkgo biloba,gene functional analysis of terpenoid synthase in Ginkgo biloba was conducted through biochemical,bioinformatics,and synthetic biology methods,providing research ideas and methods for further research on terpenoid synthase in Ginkgo biloba in the future.