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苏山猪系谱构建和群体遗传多样性分析

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摘要 苏山猪是以大白猪和苏钟猪为亲本培育而成的新品种,因疾病流行和转群等因素影响,苏山猪群体存在遗传背景不清的问题,急需重建系谱,为苏山猪持续选种选育和推广提供依据。本研究采用18个基于短散布元件(Short Interspersed Elements,SINE)的逆转录转座子插入多态(Retrotransposon Insertion Polymorphisms,RIPs)标记在苏山猪群体进行PCR鉴定,对苏山猪进行系谱重建,以亲本群体长白猪、大白猪、二花脸猪、梅山猪为参考,进行遗传参数、遗传距离、系统发育和主成分分析,以进一步探究苏山猪的遗传多样性。基于非加权组平均法(Unweighted Pair-Group Method with Arithmetic Means,UPGMA)将苏山猪群体分为10个家系,苏山猪家系的多态信息含量(Polymorphic Information Content,PIC)范围为0.1605~0.2259,遗传分化指数(Wright'sF-statistics,Fst)的范围为0.0503~0.3848,近交系数(Wright's F-statistics,Fis)的范围为-0.3894~-0.2730,而其他亲本品种猪的PIC范围为0.0580~0.2485,Fst的范围为0.410 3~0.497 9,Fis的范围为-0.098 0~0.013 1。研究表明苏山猪群体遗传多样性丰富。此研究结果为苏山猪系谱构建和推广扩繁提供了一定的理论依据。
出处 《中国畜牧杂志》 CAS CSCD 北大核心 2024年第8期168-174,共7页 Chinese Journal of Animal Science
基金 江苏省国际联合研究实验室关于国内动物种质资源与遗传改良的开放项目[IJRLD-KF202215] 江苏省种业振兴计划[JBGS(2020)028]。
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