姜DUF966基因家族成员鉴定与表达分析

Identification and Expression Pattern Analysis of DUF966 Gene Family Members in Ginger

为了解姜DUF966家族基因(ZoDUF966)的功能,利用生物学信息方法对其进行系统的鉴定。共鉴定出11个ZoDUF966,在系统发育上被划分为a、b和c组,分别包含5、4和2个成员。所有ZoDUF966蛋白均为亲水性蛋白并定位于细胞核;除ZoDUF966_1b、ZoDUF966_3b和ZoDUF966_11a外,其余8个均为碱性蛋白;蛋白质二级结构以α–螺旋和无规则卷曲为主,三级结构存在一定的差异。11个ZoDUF966分布在6条染色体上,6和16号染色体上分别有4和3个,还有4条染色体上均只有1个。ZoDUF966的启动子序列中含有大量与光响应、胁迫、生长发育以及植物激素相关的顺式作用元件。基于转录组测序数据的基因表达分析发现,ZoDUF966在不同生长时期、不同组织、生物胁迫以及非生物胁迫中的表达均存在差异,其中ZoDUF966_6c响应低温胁迫,ZoDUF966_9b则在接种腐皮镰刀菌后高度上调表达。qRT-PCR结果显示,ZoDUF966响应干旱、淹水和盐胁迫。干旱胁迫下,根系中ZoDUF966_6c/10a/11a和根茎中的Zo DUF966_7a/11a显著上调表达,根茎中ZoDUF966_1b/4b/8a/1a显著下调表达。盐胁迫下,根系中ZoDUF966_9b/7a显著上调表达,根系中ZoDUF966_5a显著下调表达。淹水胁迫下,根茎中ZoDUF966_7a和ZoDUF966_10a分别显著上调,叶中ZoDUF966_1b和根系中的ZoDUF966_11a显著下调表达。

In order to understand the function of DUF966 family genes(ZoDUF966)in ginger,the biological information method was used to systematically identify them.A total of 11 ZoDUF966 were identified and classified into groups a,b and c,including 5,4 and 2 members,respectively.All ZoDUF966 proteins were hydrophilic proteins and localized in the nucleus.Except for ZoDUF966_1b,ZoDUF966_3b and ZoDUF966_11a,the other 8 proteins were alkaline proteins.In the secondary structure of the protein,α-helix and random coil are dominant,and there are some differences in the tertiary structure.Eleven ZoDUF966 were distributed on six chromosomes,except for four and three genes on chromosomes 6 and 16,respectively,and only one gene on each of the other four chromosomes.The promoter sequence of ZoDUF966 contains cis-acting elements related to light response,stress,growth and development,and plant hormones.Gene expression analysis based on transcriptome sequencing data showed that the expression of ZoDUF966 was different in different growth periods,different tissues,biotic and abiotic stresses.Among them,ZoDUF966_6c responded to low temperature stress,and ZoDUF966_9b was highly up-regulated after inoculation with Fusarium solani.qRT-PCR results showed that ZoDUF966 responded to drought,flooding and salt stresses.Under drought stress,ZoDUF966_6c/10a/11a in roots and ZoDUF966_7a/11a in rhizomes were significantly up-regulated,while ZoDUF966_1b/4b and ZoDUF966_8a/1a in rhizomes were significantly down-regulated.Under salt stress,ZoDUF966_9b and ZoDUF966_7a were significantly up-regulated in roots,and ZoDUF966_5a was significantly downregulated in roots.Under waterlogging stress,ZoDUF966_7a and ZoDUF966_10a were significantly up-regulated in rhizome,while ZoDUF966_1b in leaves and ZoDUF966_11a in roots were significantly down-regulated.