铁皮石斛CCO基因家族鉴定及其在花发育中的表达分析

Genome-Wide Identification and Expression Analysis of CCO Gene Family in Dendrobium officinale During Flower Development

为了解铁皮石斛(Dendrobiumofficinale)中类胡萝卜素裂解氧化酶(carotenoidcleavage oxygenase,CCO)基因家族的功能,通过生物信息的方法鉴定铁皮石斛CCO家族成员,同时分析它们在不同组织、不同发育时期的花及茉莉酸甲酯(methyl jasmonate,MeJA)处理后幼苗叶片中的表达量。结果表明,铁皮石斛有12个CCO家族成员,10个属于CCD亚家族,2个属于NCED亚家族,不均匀定位于7条染色体上,且存在串联重复基因对。该家族基因启动子上存在大量激素响应和逆境响应元件。DoCCO家族基因在铁皮石斛各组织中均有表达,多数在花粉和根中高表达,它们在不同发育时期的花和MeJA处理后叶片中呈现差异化表达,可能参与不同组织的生长发育及MeJA响应,其中DoCCD1b可能在花发育中发挥重要作用。

To investigate the functions of carotenoid cleavage oxygenase(CCO)gene family in Dendrobium officinale Kimura et Migo,bioinformatics methods were employed to identify the CCO family members in D.officinale.Simultaneously,their expression levels in different tissues,different stages of flower development,and after the application of methyl jasmonate(MeJA).The results revealed that a total of 12 CCO family members were excavated,with ten belonging to the carotenoid cleavage dioxygenase(CCD)subfamily and two belonging to the 9-cis-epoxycarotenoid dioxygenase(NCED)subfamily.They were unevenly distributed on 7 chromosomes,and harbored tandem duplications.The promoter regions of these DoCCO genes contained numerous hormone and stress response elements.The expression of DoCCO genes varied across different tissues of D.officinale,with higher expression observed in pollen and roots.They were differentially expressed during flower development and in response to MeJA treatment,suggesting that their potential roles in growth,development and the MeJA response.Specifically,DoCCD1b,may play a crucial role during flower development.