结直肠癌患者血清中miR-491-3p和miR-412-3p的表达水平及其与预后的关系

The expression levels of serum miR-491-3p and miR-412-3p in patients with colorectal cancer and their relationship with prognosis

目的:探讨血清微小RNA-491-3p(miR-491-3p)和miR-412-3p的表达水平与结直肠癌(CRC)患者预后的关系及其预测价值。方法:收集110例CRC患者(CRC组)和98例体检健康受试者(对照组)作为观察对象,qRT-PCR法检测血清miR-491-3p、miR-412-3p的表达水平;根据CRC患者随访结果分为存活组73例和死亡组37例,比较其临床资料及血清miR-491-3p、miR-412-3p的表达水平;采用受试者工作特征(ROC)曲线分析CRC患者血清miR-491-3p、miR-412-3p的水平对CRC患者死亡的预测价值,多因素Cox回归分析影响CRC患者预后的因素。结果:与对照组比较,CRC组miR-491-3p表达水平降低,miR-412-3p表达水平升高(P<0.05);相较于无淋巴结转移和TNMⅠ-Ⅱ期患者,有淋巴结转移和TNMⅢ-Ⅳ期患者miR-491-3p水平降低,miR-412-3p水平升高(P<0.05);相较于存活组,死亡组患者有淋巴结转移和TNMⅢ-Ⅳ期患者比例增加,miR-491-3p表达水平降低、miR-412-3p表达水平升高(P<0.05);miR-491-3p、miR-412-3p水平单独及联合预测CRC患者3年发生死亡的AUC分别为0.842、0.917、0.967;miR-412-3p是CRC患者3年死亡的独立危险因素,miR-491-3p是保护因素(P<0.05)。结论:CRC患者miR-491-3p表达水平降低、miR-412-3p表达水平升高,都与患者预后相关,且二者对CRC患者3年预后具有一定的预测价值。

Objective:To explore the relationship between the expression levels of serum microRNA-491-3p(miR-491-3p)and miR-412-3p and the prognosis of colorectal cancer(CRC)patients and their predictive value.Methods:A total of 110 CRC patients(CRC group)and 98 healthy subjects(control group)were collected as observation objects.The expression levels of serum miR-491-3p and miR-412-3p were detected by qRT-PCR.Aaccording to the follow-up results,CRC patients were divided into survival group(73 cases)and death group(37 cases).Clinical data and serum expression levels of miR-491-3p and miR-412-3p were compared.ROC curve was applied to analyze the predictive value of serum miR-491-3p and miR-412-3p levels in mortality of CRC patients,multivariate Cox regression was applied to analyze factors that affected the prognosis of CRC patients.Results:Compared with the control group,the miR-491-3p expression level in the CRC group was decreased,while the miR-412-3p expression level was increased(P<0.05).Compared with patients without lymph node metastasis and TNM stage I-II,patients with lymph node metastasis and TNM stage III-IV hada lower miR-491-3p level and a higher miR-412-3p level(P<0.05).Compared to the survival group,the proportion of patients with lymph node metastasis and TNM stage III-IV in the death group increased,and the expression level of miR-491-3p was decreased,while the expression level of miR-412-3p was increased(P<0.05).The AUC of miR-491-3p and miR-412-3p levels,and combined in predicting 3-year mortality in CRC patients was 0.842,0.917,and 0.967,respectively.miR-412-3p was an independent risk factor for 3-year mortality in CRC patients,while miR-491-3p was a protective factor(P<0.05).Conclusion:The expression level of miR-491-3p in serum of CRC patients decreases,while the expression level of miR-412-3p increases,they are related to patients'prognosis,and both have certain predictive value for the 3-year prognosis of CRC patients.