猫、犬融合变应原Fel d 1-Can f 1过敏性哮喘小鼠模型的建立

Construction of a mouse model of allergic asthma induced by fusion of allergen Fel d 1-Can f 1 of cats and dogs

为构建可靠、稳定的过敏性哮喘小鼠模型,选取12只5周龄雌性BALB/c小鼠,随机分为模型组和对照组,每组6只。模型组每只小鼠腹腔注射100μL的1 g·L^(-1)猫、犬融合变应原Fel d 1-Can f 1/氢氧化铝复合物致敏,并经气管注入100μL的1 g·L^(-1)猫、犬融合变应原Fel d 1-Can f 1激发,对照组给予等体积PBS(pH=7.4)。检测血清特异性IgE水平、气道反应性水平、变应原诱发应激性过敏反应、耳朵点刺试验、肺部组织炎症浸润程度、肺部组织多黏液杯状细胞数和胶原纤维沉积程度等多个指标,以判断过敏性哮喘模型是否构建成功。结果表明,与对照组相比,经Fel d 1-Can f 1诱导的小鼠血清特异性IgE水平显著上升,气道反应性显著增高,变应原诱发体温下降明显,耳朵染料渗漏面积增大,渗漏净强度显著增强,肺部炎症浸润显著增加,多黏液杯状细胞数增加,胶原纤维沉积程度加重。综上所述,本研究成功构建过敏性哮喘小鼠模型,可为猫、犬过敏防治型疫苗的开发及评价提供模型条件。

In order to construct a reliable and stable mouse model of allergic asthma,125-week-old female BALB/c mice were selected and randomly divided into a model group and a control group of 6 mice each.Each mouse in the model group was sensitized with an intraperitoneal injection of 1 mg-mL^(-1) feline and canine fusion allergen Fel d 1-Can f 1/aluminum hydroxide complexes,and injected with 1 mg-mL^(-1) feline and canine fusion allergen Fel d 1-Can f 1 via the trachea.The control group was given an equal volume of PBS.A number of indices were employed to ascertain the efficacy of the constructed allergic asthma model,including serum specific IgE levels,airway reactivity levels,allergen-induced stress allergic reactions,ear prick tests,the degree of inflammatory infiltration in lung tissues,the number of polymucous cuprocytes in lung tissues,and the degree of collagen fiber deposition.The results demonstrated that mice exposed to Fel d 1-Can f 1 exhibited a notable elevation in serum-specific IgE levels,a substantial increase in airway responsiveness,a significant decline in allergen-induced body temperature,and an expansion in the area of ear dye.Furthermore,the study revealed that the mice exhibited leakage and a significant enhancement in the net intensity of leakage,as well as a significant increase in inflammatory infiltration of the lungs,an increase in the number of multilocular cup cells,and an aggravation in the degree of collagen fiber deposition,when compared to the control group.In conclusion,a mouse model of allergic asthma was successfully constructed,which will provide model conditions for the development and evaluation of allergy-control vaccines for cats and dogs.