普通大蓟马视觉基因的克隆与表达分析

Cloning and expression analysis of visual genes of Megalurothrips usitatus

普通大蓟马(Megalurothrips usitatus)是一种危害豇豆的重要害虫,具有明显的趋光(色)性,并且光环境可影响其行为和生理。本研究通过RT-PCR扩增,得到普通大蓟马4个视觉基因,并将序列上传至NCBI;另外,通过实时荧光定量PCR检测不同龄期和不同性别普通大蓟马视觉基因的表达量。结果表明,4个视觉基因均属于G蛋白偶联受体家族成员;不同龄期的普通大蓟马4个视觉基因表达量存在显著性差异(P<0.05);不同性别普通大蓟马视觉基因表达量存在显著差异(P<0.05),且雄虫的视觉基因表达量均高于雌虫。本研究明确了普通大蓟马视觉基因的分子特征,揭示了其发育过程中的变化规律,对利用蓝色黏虫板或改变光环境等方法在田间精准防控普通大蓟马具有重要指导作用。

Megalurothrips usitatus is an important thrips pest of legume crops,with obvious phototaxis(color),and the light environment can affect its behavior and physiology.Four visual genes were cloned and their sequence data were uploaded to NCBI for searching.Moreover,the expression changes of the visual genes at different ages and genders were detected by real-time PCR.The results indicate that all four visual genes belong to the G protein coupled receptor family members.There were significant differences in expression levels of four visual genes among different age groups of M.usitatus(P<0.05),and their expression levels fluctuated in different age groups.There is a significant difference in the expression level of visual genes between female and male adults(P<0.05),and the expression level of visual genes in males is higher than that in females at all ages.This study clarified the molecular characteristics of the visual genes of M.usitatus and revealed the patterns of changes during their development,which has important guiding significance for precision control of thrips in the field using methods such as blue sticky insect plates or changing the light environment.