CGF用于牙髓血管再生对内皮细胞增殖的影响

Effect of CGF on Endothelial Cell Proliferation in Dental Pulp Vascular Regeneration

目的:研究浓缩生长因子(Concentrated growth factor,CGF)用于牙髓血管再生对内皮细胞增殖的影响。方法:选取笔者医院2019年3月-2021年3月拟行牙髓血管再生的年轻恒牙牙髓坏死患儿84例作为研究对象,按照随机数表法分为CGF组和对照组,各42例。两组均行牙髓血管再生治疗,CGF组采用CGF覆盖,对照组采用无机三氧化物聚合体(Mineral trioxide aggregate,MTA)覆盖。手术6个月后观察两组临床疗效,观察术前、术后6个月牙根尺寸变化情况[牙根长度、根尖孔直径、影像学牙根区域(Radiographic root area,RRA)],观察两组术后6个月牙髓活力恢复情况,测定两组手术前后血管内皮细胞表面标记物表达情况[血小板-内皮细胞黏附分子(CD31)、血管内皮生长因子受体2(Vascular endothelial growth factor receptor 2,VEGFR2)、血管内皮钙黏蛋白(Vascularendothelial cell cadherin,VE-Cadherin)],统计两组随访期间并发症发生情况。结果:观察组总有效率为100.00%,对照组为97.62%,差异无统计学意义(P>0.05)。术后6个月,CGF组牙根长度、PRA显著高于本组术前及对照组术后6个月,根尖孔直径显著低于同组术前及对照组术后6个月(P<0.05)。CGF组患儿牙髓活力测试阳性率为40.48%(17/42),对照组为19.05%(8/40),比较差异有统计学意义(P<0.05)。术后6个月,CGF组CD31、VEGFR2、VE-Cadherin表达水平高于本组术前及对照组术后6个月(P<0.05)。两组并发症发生率比较差异无统计学意义(P>0.05)。结论:CGF用于牙髓血管再生可有效促进年轻恒牙继续发育,恢复部分牙髓活力,改善患牙内皮细胞增殖情况,值得临床推广。

Objective To study the effect of concentrated growth factor(CGF)on endothelial cell proliferation in dental pulp vascular regeneration.Methods 84 children with pulp necrosis of young permanent teeth who planned to undergo pulp vascular regeneration in the author's hospital from March 2019 to March 2021 were selected as the research objects.They were randomly divided into CGF group and control group,with 42 cases in each group.Both groups were treated with pulp vascular regeneration.The CGF group was covered with CGF and the control group was covered with inorganic trioxide polymer(MTA).The clinical effects of the two groups were observed 6 months after operation,The changes of root size[root length,apical hole diameter,imaging root area(RRA)]were observed before and 6 months after operation,The recovery of dental pulp vitality in the two groups was observed 6 months after operation,The expression of vascular endothelial cell surface markers[platelet endothelial cell adhesion molecule(CD31),vascular endothelial growth factor receptor 2(VEGFR2),vascular endothelial cadherin]before and after operation were measured,the complications of the two groups during follow-up were counted.Results The total effective rate was 100.00%in the observation group and 97.62%in the control group,with no significant difference(P>0.05).6 months after operation,the root length and pra of CGF group were significantly higher than those before operation and 6 months after operation in the control group,and the diameter of apical foramen was significantly lower than that before operation and 6 months after operation in the control group(P<0.05).The positive rate of dental pulp viability test in CGF group was 40.48%(17/42)and that in control group was 19.05%(8/40).The difference was statistically significant(P<0.05).The expression levels of CD31,VEGFR2 and ve cadherin in CGF group were higher than those before operation and 6 months after operation in control group(P<0.05).There was no significant difference in the incidence of complications between the two groups(P>0.05).Conclusion CGF used in dental pulp vascular regeneration can effectively promote the continuous development of young permanent teeth,restore the vitality of some dental pulp,and improve the proliferation of endothelial cells of affected teeth,which is worthy of clinical promotion.