小果冬青组织培养和瓶外生根关键因子研究

Key factors for tissue culture and ex vitro rooting of Ilex micrococca

为建立小果冬青组织培养高效繁育技术体系,该研究分析了诸多因子对组织培养苗增殖及其瓶外生根情况的影响。通过正交试验,研究生长调节剂种类、质量浓度和蔗糖含量对小果冬青苗增殖系数的影响以及生根剂种类、质量浓度、处理时间和基质类型对小果冬青组织培养苗瓶外生根的影响。结果表明:影响小果冬青茎段芽增殖的主要因素为6-BA和蔗糖含量,较佳配比方案:6-BA质量浓度为0.5 mg/L,NAA质量浓度为0.1 mg/L,蔗糖含量为40 g/L,此时小果冬青组织培养苗增殖系数为4.52。影响小果冬青瓶外生根效果的主次顺序为基质类型、生根剂处理时间、生根剂质量浓度、生根剂种类。选用生根剂种类为IBA,质量浓度为200 mg/L,处理时间为1 min,基质为泥炭,此时小果冬青组织培养苗瓶外生根率为84.44%,生根数5.36,平均根长3.78 cm。

To establish an efficient propagation system for Ilex micrococca tissue culture,the effects of key factors on the proliferation of tissue culture seedlings and the ex vitro rooting of micro-shoots were analyzed.We studied the effects of exogenous hormone type,concentration,and sucrose content on the proliferation coefficient,and the effects of rooting agent type,concentration,processing time,and substrate type on the ex vitro rooting.The results showed that the dominant factors affecting the proliferation were 6-BA concentration and sucrose concentration.The optimal solution was 6-BA concentration of 0.5 mg/L,NAA concentration of 0.1 mg/L,and sucrose concentration of 40 g/L.Under this condition,the proliferation coefficient was 4.52.The factors that affected the ex vitro rooting effect were,inoder,substrate type,agent processing time,concentration and agent type.The preferable conditions of ex vitro rooting of the micro-shoots were that after pretreated with IBA concentration 200 mg/L,processing time 1 minute in the peat substrate,84.44%of rooting rate,5.36 of average root number and 3.78 cm of average root length could be gotten.