抑瘤素M在子宫内膜癌中的表达及其与免疫细胞浸润的相关性分析

Oncostatin M expression in endometrial cancer and its correlation with immune cell infiltration

目的:探索抑瘤素M(OSM)在子宫内膜癌中的表达及预后价值,分析OSM表达与子宫内膜癌组织免疫细胞浸润的关系。方法:TIMER数据库分析OSM在泛癌中的表达,比较OSM在子宫内膜癌与正常组织的表达,并对不同OSM表达患者进行生存分析;TIMER和TISIDB分析OSM表达与免疫细胞浸润的关系,ssGSEA算法计算不同OSM表达样本免疫细胞浸润丰度差异;GSEA软件进行富集分析;收集临床组织样本进行验证。结果:OSM在子宫内膜癌组织中的表达高于正常子宫内膜组织(P=4.1e-28),且OSM高表达子宫内膜癌患者无复发生存期(RFS)延长(P=0.004 8)。OSM表达与免疫细胞浸润丰度和免疫细胞基因标志物均呈正相关(P<0.05)。OSM主要富集于免疫相关信号通路。OSM在子宫内膜癌组织中的表达高于正常和不典型增生组织(P=0.016 9)。OSM高表达肿瘤组织中,免疫细胞标志物CD4、CD8、CD68比例升高(均P<0.05),OSM表达与CD4、CD8、CD68均呈显著正相关。结论:OSM在子宫内膜癌组织中高表达且与预后相关;OSM表达与免疫细胞浸润水平呈正相关,可作为免疫治疗和预后判断的生物标志物。

Objective:To explore expression and prognostic value of oncostatin M(OSM)in endometrial cancer and to analyze relationship between OSM expression and immune cell infiltration in endometrial cancer tissues.Methods:OSM expression in pan-can⁃cer was analyzed by TIMER database,OSM expression in endometrial cancer and normal tissues was compared,and survival analysis for patients with different OSM expression was performed;relationship between OSM expression and immune cell infiltration was analyzed by TIMER and TISIDB,and ssGSEA algorithm was used to calculate difference in abundance of immune cell infiltration in samples with different OSM expression;GSEA software was applied to perform enrichment analysis;clinical tissue samples were collected for validation.Results:OSM expression was higher in endometrial cancer tissues than that in normal endometrial tissues(P=4.1e-28),and endometrial cancer patients with high OSM expression had prolonged recurrence-free survival(RFS)(P=0.0048).OSM expression was positively correlated with abundance of immune cell infiltration and genetic markers of immune cells(P<0.05).OSM was mainly enriched in immune-related signaling pathways.OSM expression was higher in endometrial cancer tissues than normal and atypical hyperplastic tissues(P=0.0169).Proportions of immune cell markers CD4,CD8,and CD68 were increased in tumor tissues with high OSM expression(all P<0.05),which were positively correlated with OSM expression.Conclusion:OSM is highly expressed in endometrial cancer tissues and correlated with prognosis;OSM expression is positively correlated with immune cell infiltration level and can be used as a biomarker for immunotherapy and prognosis.