红花多糖治疗雌二醇诱导的小鼠胸腺萎缩的药效作用研究

Rapeutical effect of safflower polysaccharide on thymic atrophy induced by estradiol in mice

目的:雌二醇诱导小鼠胸腺萎缩模型,评价红花多糖治疗胸腺萎缩的药效。方法:75只雌性ICR小鼠分为5组:正常对照组、模型组、乌苯美司组、SPS高、低剂量组,除对照组外,其余各组隔天腹腔注射苯甲酸雌二醇注射液,共6次;药物治疗组于末次注射24 h后开始给药,1次/d,共10 d。末次给药24 h后牺牲小鼠,观察体质量、免疫器官指数、血浆MDA与GST水平、外周血细胞与T细胞变化,并对胸腺组织进行HE染色、TUNEL细胞凋亡染色,检测胸腺输出能力。结果:高、低剂量红花多糖均可显著提高小鼠胸腺指数(P<0.05),提高外周血白细胞数量(P<0.05)、CD3^(+)CD4^(+)T、CD3^(+)CD8^(+)T细胞比例(P<0.05)及CD4^(+)T/CD8^(+)T,并改善胸腺组织损伤。高剂量红花多糖可显著减少胸腺组织细胞凋亡,并提高胸腺输出能力。结论:红花多糖对雌二醇诱导的小鼠胸腺萎缩具有治疗作用。

Objective:To evaluate efficacy of safflower polysaccharide in treatment of thymic atrophy induced by estradiol in mice.Methods:A total of 75 female ICR mice were divided into 5 groups:control group,model group,ubenimex group,SPS high dose group,SPS low dose group.Except for control group,each group was given intraperitoneal injection of estradiol benzoate every other day for 6 times.Treatment group began administration 24 h after the last parenteral administration,once a day for 10 days.Mice were sacrificed 24 hours after the last administration,and body weight,immune organ index,MDA and GST levels in plasma,periph⁃eral blood cells and T cells changes were observed,thymus tissue was stained by HE staining and TUNEL cell apoptosis staining,and thymus output capacity was detected.Results:Both high and low doses of safflower polysaccharide significantly improved thymus index(P<0.05)in mice,increased leukocytes level in peripheral blood(P<0.05),proportions of CD3^(+)CD4^(+)T,CD3^(+)CD8^(+)T cells(P<0.05)and CD4^(+)T/CD8^(+)T,improved thymus tissue damage.High dose of safflower polysaccharide could significantly reduce apoptosis in thymus tissue and enhance thymus output.Conclusion:Safflower polysaccharide has a certain therapeutic effect on estradiol-induced thymus atrophy in mice.