血清miR-135b、LZST1表达水平与晚期非小细胞肺癌患者预后的相关性

Correlation of Serum miR-135b and LZST1 Expression Levels and Prognosis in Patients with Advanced Non-small Cell Lung Cancer

目的探究晚期非小细胞肺癌(NSCLC)患者血清微小RNA-135b(miR-135b)、亮氨酸拉链肿瘤抑制基因1(LZST1)表达水平及与其预后的相关性。方法收集62例晚期NSCLC患者作为观察组。另外收集同时期94例良性肺部病变患者作为对照组。比较观察组与对照组血清miR-135b、LZST1表达水平差异,比较不同预后患者血清miR-135b、LZST1表达水平差异,分析晚期NSCLC患者血清miR-135b、LZST1表达水平与其预后的关系,miR-135b、LZST1对晚期NSCLC患者预后不良的预测价值。结果两组性别、年龄、体质量指数比较差异不显著,P>0.05。观察组血清miR-135b表达水平高于对照组,LZST1低于对照组,差异有统计学意义(P<0.05)。死亡患者血清miR-135b表达水平高于存活患者,LZST1水平低于对照组,差异有统计学意义(P<0.05)。miR-135b与晚期NSCLC患者死亡呈显著正相关关系,LZST1与晚期NSCLC患者死亡呈负相关关系,miR-135b与LZST1表达呈负相关关系,P<0.05。计算约登指数,获取最大约登指数相对应的临界值。4.065为miR-135b预测晚期NSCLC患者死亡的阈值,灵敏度84.00%,特异度81.10%,0.670为LZST1预测晚期NSCLC患者死亡的阈值,灵敏度68.00%,特异度78.40%。miR-135b、LZST1以及两者联合的AUC面积分别为0.792、0.742以及0.850,两者联合的AUC面积最大,灵敏度为88.00%,特异度为70.30%,预测效能较好。结论晚期NSCLC患者血清miR-135b表达水平异常升高,LZST1显著降低,miR-135b高表达、LZST1低表达与患者预后不良相关,miR-135b与LZST1呈负相关关系。miR-135b联合LZST1对晚期NSCLC患者死亡的预测效能较高。

Objective To investigate the expression levels of serum micrornA-135b(miR-135b)and leucine zipline tumor suppressor gene 1(LZST1)in patients with advanced non-small cell lung cancer(NSCLC)and their correlation with prognosis.Methods 62 patients with advanced NSCLC were collected as the observation group.In addition,94 patients with benign pulmonary lesions were collected as control group.Compare the expression levels of serum miR-135b and LZST1 between the observation group and the control group,compared the expression levels of serum miR-135b and LZST1 in patients with different prognosis,and analyze the relationship between the expression levels of serum miR-135b and LZST1 in patients with advanced NSCLC and their prognosis.Prognostic value of miR-135b and LZST1 in patients with advanced NSCLC.Results There were no significant differences in gender,age and body mass index between the 2 groups(P>0.05).The expression level of serum miR-135b in the observation group was higher than that in the control group,and LZST1 was lower than that in the control group,the difference was statistically significant(P<0.05).The serum miR-135b expression level of dead patients was higher than that of surviving patients,and the LZST1 level was lower than that of the control group,with statistical significance(P<0.05).There was a significant positive correlation between miR-135b and death in advanced NSCLC patients,a negative correlation between LZST1 and death in advanced NSCLC patients,and a negative correlation between miR-135b and LZST1 expression(P<0.05).Calculate the Jorden index and obtain the critical value corresponding to the maximum approximate index.4.065 was the threshold for miR-135b to predict death in patients with advanced NSCLC,with a sensitivity of 84.00%and a specificity of 81.10%;0.670 was the threshold for LZST1 to predict death in patients with advanced NSCLC,with a sensitivity of 68.00%and a specificity of 78.40%.The AUC area of miR-135b,LZST1 and their combination was 0.792,0.742 and 0.850,respectively.The AUC area of Mir-135b,LZST1 and their combination was the largest,the sensitivity was 88.00%,and the specificity was 70.30%,indicating good prediction efficiency.Conclusion The expression level of serum miR-135b is abnormally elevated and LZST1 is significantly decreased in patients with advanced NSCLC.High expression of miR-135b and low expression of LZST1 are correlated with poor prognosis of patients,and miR-135b is negatively correlated with LZST1.miR-135b combined with LZST1 has high predictive efficacy in patients with advanced NSCLC.