益生性酵母的分离鉴定与安全性评价

Isolation identification and safety evaluation of probiotic yeast

试验旨在筛选益生性酵母菌,为后续酵母菌作为饲料添加剂提供依据。试验从兰州醪糟和甜胚子中分离筛选酵母菌,并对其进行益生特性和安全性评价。结果表明,从兰州醪糟和甜胚子中获得到库德里阿兹威氏毕赤酵母2株、葡萄牙棒孢酵母1株、酿酒酵母1株、马克斯克鲁维酵母1株。其中,菌株JM-4发酵完成时间短,环境适应性强,生命力旺盛,可以在肠液胃液中生存,具有良好的生长特性和益生特性。同时,该菌株在小鼠体内无明显的毒性作用,安全性良好。研究表明,5种试验酵母菌株对小鼠具有良好的安全性,适合用于益生菌制剂的制备,为酵母菌作为饲料添加剂提供了参考。

The experiment aimed to screen probiotic yeast and provide a basis for subsequent yeast use as feed additives.Use yeast enrichment medium to isolate and screen yeast from Lanzhou fermented grains and sweet embryos sold,and evaluate their probiotic properties and safety.The results showed that two strains of Pichia kudriavzevii,one strain of Clavisporalusitaniaefrom Portugal,one strain of Saccharomycescerevisiae,and one strain of Kluyveromycesmarxianus were obtained from Lanzhou fermented grains and sweet embryo.Among them,strain JM-4 had a short fermentation completion time,strong environmental adaptability,and strong vitality.It could survive in intestinal and gastric fluids and had good growth and probiotic properties.At the same time,this strain had no obvious toxic effects in mice,displaying good security.The research indicates that the five experimental strains of yeast show good safety for mice,making them suitable for the preparation of probiotic formulations,providing a reference for the use of yeast as feed additives.