解毒活血汤对慢性肾脏病(3-5期)大鼠肾脏组织与血管钙化影响的研究

Study on the Effect of Jiedu Huoxue Decoction on Renal Tissue and Vascular Calcification in Chronic Kidney Disease(Stages 3-5)Rats

目的:研究解毒活血汤对慢性肾脏病大鼠肾脏、血清钙与胸主动脉管壁钙含量的影响。方法:72只SPF级健康雄性SD大鼠,随机分为空白组、模型组、骨化三醇组、解毒活血汤低剂量组、解毒活血汤中剂量组、解毒活血汤高剂量组,每组12只。采用腺嘌呤加高磷饮食制作慢性肾脏病大鼠伴血管钙化模型。采用生化试剂盒测定血清中肌酐(Scr)、钙(Ca)水平;HE染色观察肾脏组织病理;使用甲基百里香酚蓝检测血管壁钙含量。结果:与模型组相比,解毒活血汤高剂量组肌酐降低(P<0.05),血钙上升(P<0.05),组织钙含量下降(P<0.05)。HE染色结果显示,模型组与骨化三醇组大鼠肾脏组织肾小球结构受到破坏,与周围组织边界不清,肾小管直径扩大,其中存在坏死脱落的上皮细胞,肾间质水肿,存在炎性细胞浸润,其余各组大鼠的肾脏结构均有不同程度的损害;与模型组相比,骨化三醇组与解毒活血汤各剂量组大鼠肾脏组织结构均有不同程度的恢复。结论:解毒活血汤能改善慢性肾脏病(CKD)后期大鼠的肾脏功能,减轻肾脏结构的破坏,抑制血管钙化。

Objective:To investigate the effects of Jiedu Huoxue Decoction on renal tissue,serum calcium,and thoracic aorta wall calcium content in rats with chronic kidney disease(CKD).Methods:Seventy-two SPF-grade healthy male SD rats were randomly divided into the Control group(Group A),Model group(Group B),Calcitriol group(Group C),Jiedu Huoxue Decoction low-dose group(Group I),medium-dose group(Group H),and high-dose group(Group G),with 12 rats in each group.A CKD model with vascular calcification was established using adenine and high-phosphorus diet.Serum creatinine(Scr)and calcium(Ca)levels were measured using biochemical kits;renal tissue pathology was observed by HE staining;and vascular wall calcium content was determined by methylthymol blue.Results:Compared with the Model group,the high-dose group of Jiedu Huoxue Decoction showed reduced creatinine(P<0.05),increased serum calcium(P<0.05),and decreased tissue calcium content(P<0.05).HE staining results indicated that in the Model group and Calcitriol group,the renal glomerular structure was damaged,with blurred boundaries between the boundary tissues,enlarged renal tubule diameters,necrotic and sloughed epithelial cells,renal interstitial edema,and inflammatory cell infiltration.The renal structure was impaired to varying degrees in the other groups.Compared with the Model group,the renal tissue structure of the Calcitriol group and all Jiedu Huoxue Decoction dose groups showed varying degrees of recovery.Conclusion:Jiedu Huoxue Decoction can improve renal function in late-stage CKD rats,alleviate renal structural damage,and inhibit vascular calcification.