马铃薯StDREBs基因的克隆及其表达分析

Cloning and Expression Analysis of StDREBs Gene in Solanum tuberosum L.

【目的】探究马铃薯中OsDREB1C同源基因StDREBs在低氮环境下的表达特性,以评估其是否具备提高马铃薯氮利用效率的潜力,为后续基因功能验证及作物改良提供理论依据。【方法】通过同源克隆获得OsDREB1C在马铃薯(Solanum tuberosum L.)中的同源基因,发现马铃薯中共有4个OsDREB1C的同源基因,对其进行生物信息学、亚细胞定位、低氮条件下的表达量分析。【结果】马铃薯中的StDREBs蛋白含有AP2保守结构域,蛋白产物均定位在细胞核中;StDREBs基因在根、茎、叶中均表达,在根中具有较高的表达量,在茎中其次,在叶片中的相对表达量最低。缺氮处理下,不同组织中的StDREBs基因表达量随着处理时间增加而上升,其中1/10 N处理18 h时,StDREBs基因的表达量达到峰值,表明StDREBs在马铃薯中参与对低氮环境的响应。【结论】马铃薯中OsDREB1C同源基因(StDREBs)在马铃薯低氮胁迫响应中起关键作用。

【Objective】This study is aimed to investigate the expression characteristics of OsDREB1C homologous genes(StDREBs)in potato(Solanum tuberosum L.)under low nitrogen conditions,evaluating their potential to enhance nitrogen use efficiency in potato,thereby providing a theoretical basis for subsequent gene functional validation and crop improvement.【Method】The homologous gene of OsDREB1C in potato was obtained by homologous cloning.Four homologous genes of OsDREB1C were found in the potato.Their expressions were analyzed via bioinformatics,subcellular localization and low nitrogen conditions.【Result】StDREBs protein in potato contained AP2 conserved domain,and the protein products were located in the nucleus.StDREBs gene was expressed in the roots,stems and leaves,with a higher expression in the roots,followed by in the stems,and the lowest relative expression in the leaves.Under nitrogen deficiency treatment,the expressions of StDREBs gene in different tissues increased with the increasing of treatment time,and the expression of StDREBs gene reached its peak when 1/10N was treated for 18 h,indicating that StDREBs participated in the response to low-nitrogen environment in potato.【Conclusion】OsDREB1C homologs(StDREBs)play a key role in potato under low-nitrogen conditions.