马铃薯AQP基因家族鉴定及表达分析

Identification and Expression Analysis of AQP Gene Family in Potato

【目的】水通道蛋白(Aquaporins,AQPs)在植物非生物胁迫下的植物生长发育、水分运输和应激反应等生理过程中至关重要,本研究旨在揭示马铃薯StAQP家族的特性及功能。【方法】对马铃薯StAQP基因家族进行全基因组鉴定,利用生物信息学手段对基因结构、保守基序、顺式作用元件、染色体定位以及基因共线性等进行分析,并结合转录组数据以及RT-qPCR对基因表达模式进行验证。【结果】马铃薯StAQP基因家族的44个成员分布在PIP、TIP、NIP、SIP和XIP五个亚族中,相同亚族的家族成员的基因结构和保守基序基本一致,同时马铃薯AQP家族成员大多数分布在质膜上;马铃薯StAQP家族成员中含有大量和光反应以及胁迫响应有关的顺式作用元件;共线性结果表明,马铃薯AQP家族与拟南芥、烟草、番茄和辣椒4个物种的AQP家族之间存在大量同源基因对;转录组和RT-qPCR验证结果表明,马铃薯StAQP家族成员在干旱胁迫和盐胁迫下高度表达,不同家族成员表达存在差异,同时相同基因在不同胁迫下的表达情况也存在差异,其中StAQP7、StAQP28和StAQP31这3个基因是StAQP家族响应非生物胁迫的关键基因。【结论】StAQPs基因在马铃薯的生长发育和非生物胁迫响应中发挥重要作用。

【Objective】Aquaporins(AQPs)are crucial in physiological processes such as plant growth and development,water transport and stress response under abiotic stress in plants,and the present study was aimed to reveal the properties and functions of the potato StAQP family.【Method】The potato's StAQP gene family was identified by genome-wide,and their gene structures,conserved motifs,cis-acting progenitors,chromosomal localization,and gene collinearity were analyzed using bioinformatics,and the gene expression patterns were verified by combining transcriptome data and RT-qPCR.【Result】The 44 members of the potato StAQP gene family were distributed in five subfamilies:PIP,TIP,NIP,SIP and XIP.The gene structures and conserved motifs of the members in the same subfamily were basically the same,and the most of the members of the potato AQP family were distributed in the plasma membrane;the members of the potato StAQP family contained a large number of cis-acting progenitors related to light and stress response.The results of collinearity analysis showed that there were a large number of homologous gene pairs in AQP family between the potato and Arabidopsis thaliana,Nicotiana attenuata,Solanum lycopersicu and Capsicum annuum.The results of transcriptome and RT-qPCR validation showed that the potato's StAQP family members were highly expressed under drought and salt stress,and that there was a difference in the expressions of different members under different stresses.Differences in the expression of the same genes under different stresses also existed,three of these genes,StAQP7,StAQP28 and StAQP31,were the key genes of the StAQP family in response to abiotic stresses.【Conclusion】StAQPs genes play an important role in the growth and development of potato and in the response to abiotic stresses.