腐植酸钾协同微生物菌剂对矿区废弃地土壤改良的影响

Effects of Combined Applications of Potassium Humate with Different Microbial Agents on Soil Improvement of Mine Wasteland

为研究腐植酸钾(HAK)混合搭配施用哈茨木霉菌(TH)、胶质芽孢杆菌(BM)与细黄链霉菌(SM)在土壤改良中的效果,设置单施HAK、单菌+HAK混施、双菌+HAK混施及三菌+HAK混施处理及对照共9个处理,进行盆栽试验,测定土壤理化性质和土壤酶活性指标,并以灰色关联度理论对土壤性质改良效果进行排名。结果表明:单施HAK显著提升土壤含水率与磷酸单酯酶活性,HAK与菌剂混合施用对土壤改良效果优于单施HAK处理。其中,单菌+HAK处理中BM+HAK处理较空白对照增加全氮、有效磷含量,并提高土壤脲酶活性,且较单施HAK处理增加土壤碱解氮、有机质含量,TH+HAK处理较单施HAK处理提高土壤蔗糖酶、磷酸单酯酶活性,SM+HAK处理较空白对照脲酶活性明显下降;双菌+HAK处理中TH+BM+HAK处理较BM+HAK处理显著增加土壤碱解氮、有效磷含量,但对土壤酶活性提升作用介于TH+HAK处理与BM+HAK处理之间;三菌+HAK处理对土壤改善能力介于单菌+HAK处理与双菌+HAK处理之间。灰色关联度分析结果显示,BM+HAK处理加权关联度最高,为0.972,综合改良效果最佳,可作为土壤质量提升的一种优化改良组合。

In order to study the effects of Trichoderma harzia(TH),Bacillus glia(BM)and Streptomyces flavus(SM)mixed with potassium humate(HAK)on soil improvement,9 treatments including 1)HAK,2)TH+HAK,3)BM+HAK,4)SM+HAK,6)TH+BM+HAK,7)TH+SM+HAK,8)BM+SM+HAK,and 9)TH+BM+SM+HAK were set up for a pot experiment.Soil physicochemical and enzyme activities were measured,and soil improvement effects were ranked by grey correlation degree theory.The results showed that single HAK significantly improved soil moisture and phosphomonesterase activity,but its effects were lower than other treatments(different bactericide+HAK).In single bacteria+HAK,BM+HAK increased the contents of total nitrogen and available phosphorus and the activities of soil urease compared with the blank control,and increased the content of soil alkali-hydrolytic nitrogen and organic matter compared with single HAK,TH+HAK increased the activities of soil sucrase and phosphomonesterase compared with single HAK,and SM+HAK significantly decreased the activity of urease compared with the blank control.Compared with BM+HAK,TH+BM+HAK significantly increased the contents of alkali-hydrolytic nitrogen and available phosphorus,but its effects in increasing soil enzyme activities were between those of TH+HAK and BM+HAK.The ability of three bacteria+HAK in improving soil was between single and double bacteria+HAK.According to the results of grey correlation degree analysis,the weighted correlation degree of BM+HAK was the highest(0.972).In conclusion,BM+HAK has the best improvement effect and can be used as the optimal combination for soil quality improvement.