光叶兔耳风石油醚部位化学成分和抗菌活性研究

Chemical Constituents from Petroleum Ether Fractions of Ainsliaea glabra and Antibacterial Activities

为了解兔耳风(Ainsliaea glabra)的化学成分,采用硅胶、凝胶(Sephadex LH-20)柱色谱、半制备高效液相色谱等方法,从其90%乙醇提取物的石油醚萃取部位分离了10个化合物,根据波谱数据分别鉴定为松柏烯醇二异戊酸酯(1)、3β-hydroxy-11α,13-dihydro-costunolide(2)、matricarin(3)、乙酰伪蒲公英甾醇(4)、白桦脂醇(5)、27-hydroxyolean-12-en-28-oic acid(6)、morolic acid acetate(7)、异黑麦草内酯(8)、苯甲酸丁酯(9)和E-phytenal(10),其中化合物1为新结构化合物,化合物3和7首次在兔耳风属中分离得到,化合物2~7、9均为首次在光叶兔耳风报道。抗菌活性测试结果表明,化合物1、2、8对金黄色葡萄球菌有一定抑制作用,MIC值分别为31.2、25.0、21.8μg/m L,化合物1和3对白色念珠菌有潜在抑制作用,MIC值分别为1.9和3.9μg/m L。

In order to understand the chemical composition of Ainsliaea glabra,ten compounds were isolated from petroleum ether extraction fraction of 90%ethanol extract by silica gel,sephadex LH-20 gel column chromatography,semi-preparative high-performance liquid chromatography.Based on spectral data,their structures were identified as pinerenol diisovalerate(1),3β-hydroxy-11α,13-dihydro-costunolide(2),matricarin(3),taraxasteryl acetate(4),betulyl alcohol(5),27-hydroxyolean-12-en-28-oic acid(6),morolic acid acetate(7),(-)-loliolide(8),butyl benzoate(9),and E-phytenal(10).Compound 1 was a new structural compound.Compounds 3 and 7 were isolated from Ainsliaea genus for the first time.Compounds 2-7 and 9 were first reported in the A.glabra.The results of antibacterial activity test showed that compounds 1,2 and 8 had certain inhibitory effects on Staphylococcus aureus with MIC values of 31.2,25.0,and 21.8μg/mL,and compounds 1 and 3 had potential inhibitory effect on Candida albicans with MIC values of 1.9 and 3.9μg/mL,respectively.