西红花酸通过PI3K/Akt通路抑制结肠癌细胞恶性发展

Crocetin inhibits the malignant development of colon cancer cells through PI3K/Akt pathway

目的探讨西红花酸能否通过PI3K/Akt通路抑制结肠癌细胞恶性发展。方法采用西红花酸处理结肠癌HT29和SW480细胞系,分为对照组(未处理HT29和SW480细胞,NC组)和西红花酸组(经西红花酸处理的HT29和SW480细胞,Cro组)。利用平板克隆实验、Annexin V-FITC/PI双染法检测实验、划痕实验及Transwell小室实验检测西红花酸对HT29和SW480细胞增殖、凋亡、迁移和侵袭的影响,利用Western blot实验检测细胞PI3K、p-PI3K、Akt与p-Akt蛋白的表达。结果与对照组相比,给予西红花酸处理后,结肠癌HT29和SW480细胞的增殖、迁移和侵袭能力显著降低,细胞凋亡率明显升高,结肠癌细胞p-PI3K与p-Akt蛋白的表达明显降低(P<0.05)。结论西红花酸可通过抑制PI3K/Akt通路抑制结肠癌细胞增殖、迁移和侵袭,促进凋亡。

Objective To investigate whether crocetin can inhibit the malignant development of colon cancer cells through PI3K/Akt pathway.Methods Colon cancer HT29 and SW480 cell lines were treated with crocetin and divided into control group(untreated HT29 and SW480 cells,NC group)and crocetin group(HT29 and SW480 cells treated with crocetin,Cro group).The effects of crocetin on proliferation,apoptosis,migration and invasion of HT29 and SW480 cells were determined by plate cloning assay,Annexin V-FITC/PI double staining assay,scrape assay and Transwell assay.The expressions of PI3K,p-PI3K,Akt and p-Akt were detected by Western blot.Results Compared with the control group,after treatment with crocetin,the proliferation,migration and invasion ability of HT29 and SW480 cells were significantly decreased,the apoptosis rate was significantly increased,and the expressions of p-PI3K and p-Akt protein in colon cancer cells was significantly decreased(P<0.05).Conclusion Crocetin can inhibit the proliferation,migration and invasion of colon cancer cells and promote apoptosis by inhibiting PI3K/Akt pathway.