蚕丝含油率微波萃取试验方法探讨

Experimental study on oil content of silk by microwave extraction

针对目前蚕丝含油率检测效率低、耗时长、资源浪费大的问题,选用微波萃取工艺,设计4因素3水平的正交试验,分析比较微波萃取法与索式萃取法在蚕丝含油率检测方面的应用,探讨微波萃取法的测试条件。试验结果表明:微波萃取法相比索式萃取法检测准确度更高,过程更环保,且检测时间和试剂用量更少;微波萃取温度、萃取时间、萃取功率及乙酸乙酯用量各因素对样品含油率测试结果的影响从大到小依次为萃取温度、乙酸乙酯用量、萃取时间和萃取功率。最优检测条件为:萃取温度67℃,乙酸乙酯用量40 mL,萃取时间15 min,萃取功率800 W。

To address the challenges of low efficiency,extended processing time,and significant resource waste in silk oil content determination,this study implemented a microwave extraction technique.A four-factor,three-level orthogonal experiment was conducted to evaluate and contrast the performance of microwave extraction and Soxhlet extraction methods in assessing the oil content of silk,with a focus on optimizing the conditions for microwave extraction.The experimental findings demonstrated that,in comparison to Soxhlet extraction,the microwave extraction method provides superior accuracy,is more environmentally sustainable,and reduces both processing time and reagent usage.A detailed analysis of the factors influencing oil content determination extraction temperature,extraction time,extraction power,and ethyl acetate volume revealed the following order of impact:extraction temperature,ethyl acetate volume,extraction time,and extraction power.The optimal extraction conditions were identified as follows:extraction temperature of 67℃,ethyl acetate volume of 40 mL,extraction time of 15 minutes,and extraction power of 800 W.