基于GC-MS联用技术对不同产地不同部位连钱草挥发性成分的比较分析

Comparison analysis of volatile compositions in different parts of Glechoma longituba from different origins based on GC-MS coupled technology.

目前,对不同产地连钱草比较研究较为缺乏,本试验旨在探究不同产地连钱草茎叶中挥发性成分的差异。采用水蒸气蒸馏法分别提取挥发性成分,利用气相色谱-质谱(GC-MS)方法,运用主成分分析(PCA)和聚类分析(CA)分析其成分的差异。由GC-MS数据可知,皖产叶、皖产茎、苏产叶、苏产茎、豫产叶和豫产茎中分别鉴定出63、46、65、38、64和27种提取的挥发性成分,共鉴定出105种,特有成分分别有12、3、9、1、15和3种,所共有挥发性成分有15种。对不同产地连钱草的叶中挥发性成分含量进行比较,未发现一致突出的化合物;而对于不同产地连钱草的茎中以棕榈酸含量明显突出。聚类分析显示,苏产茎和豫产茎挥发性物质较为相似,而与皖产茎有一定差异。分析结果表明,不同产地连钱草茎叶的挥发性成分和含量存在一定的差异,其中既有共有成分,也存在特有成分,其中棕榈酸含量都尤为突出。本研究结果可为连钱草进一步药用、饲料工业开发和畜牧疾病的治疗以及更多药理活性的发现提供参考。

Based on the scarcity of research on Glechoma longituba from different origins,this study aimed to investigate the differences of volatile components in the stems and leaves of Glechoma longituba from different origins,extract the volatile components by water vapor distillation,and analyze the differences of their components by gas chromatography-mass spectrometry(GC-MS),principal component analysis(PCA),cluster analysis(CA),and cluster analysis(CA).GC-MS,PCA,CA were used to analyze the differences in the compositions.From the GC-MS data,63,46,65,38,64 and 27 extracted volatile components were identified in Anhui leaves,Anhui stems,Jiangsu leaves,Jiangsu stems,Henan leaves and Henan stems,respectively,with a total of 105 identified,12,3,9,1,15 and 3 endemic components,respectively,and 15 volatile components shared by all.Comparison of the content of volatile components in the leaves of Glechoma longituba from different origins did not reveal any consistently prominent compounds,while the content of palmitic acid was clearly prominent in the stems of Glechoma longituba from different origins.Cluster analysis showed that the volatile substances of stems from Suzhou and Yu were more similar,while there were some differences with stems from Anhui.The results showed that the volatile components and contents of Glechoma longituba stems and leaves from different places of origin differed to some extent,with both common and specific components,among which palmitic acid content was particularly prominent.The results of this study may provide reference for further medicinal use of Glechoma longituba,development of feed industry and treatment of livestock diseases and discovery of more pharmacological activities.