日粮添加α-硫辛酸对肉牛生长性能、肉品质和肌肉抗氧化性能的影响

Effects of dietaryα-lipoic acid supplementation on growth performance,meat quality and muscle antioxidant performance of beef cattle

文章旨在探究日粮添加α-硫辛酸(α-LA)对肉牛生长性能、肉品质和肌肉抗氧化性能的影响。试验将健康的80头体重(390.47±28.31)kg的西杂肉牛随机分为4组,每组20头,每个重复1头。试验日粮分别向基础日粮中添加0、150、300和600 mg/kg的α-LA。试验为期100 d,其中包括一周的适应期。结果表明,与对照组相比,添加300和600 mg/kg的α-LA组肉牛末体重(FBW)分别显著提高2.59%和1.80%(P<0.05);添加150~600 mg/kg的α-LA组肉牛日增重(ADG)分别显著提高7.79%、9.37%和7.22%(P<0.05);添加150~600 mg/kg的α-LA组肉牛料重比(F/G)分别显著降低5.94%、7.57%和6.18%(P<0.05);此外,α-LA对肉牛平均日采食量(ADFI)无显著影响(P>0.05)。与对照组相比,添加150~600 mg/kg的α-LA组肉牛牛肉pH和红度显著提高(P<0.05);添加150~600 mg/kg的α-LA组肉牛牛肉黄度显著降低(P<0.05);添加150~600 mg/kg的α-LA组肉牛牛肉剪切力显著降低(P<0.05);此外,α-LA对肉牛牛肉的亮度、滴水损失和蒸煮损失均无显著影响(P>0.05)。与对照组相比,添加300和600 mg/kg的α-LA组肉牛背最长肌中Keap1基因表达显著降低(P<0.05);添加150~600 mg/kg的α-LA组肉牛背最长肌中SOD基因表达显著提高(P<0.05);添加150~600 mg/kg的α-LA组肉牛后腿肌Keap1基因表达显著降低(P<0.05);添加300和600 mg/kg的α-LA组肉牛后腿肌中Nrf-2基因表达显著提高(P<0.05);添加600 mg/kg的α-LA组肉牛后腿肌中SOD基因表达显著提高(P<0.05)。结论:补充α-LA能促进肉牛生长,此外,α-LA可通过调节牛肉中抗氧化基因表达改善肉质,在本试验条件下,α-LA在肉牛料中最适添加量为300 mg/kg。

To investigate the effects of dietaryα-lipoic acid supplementation on growth performance,meat quality and muscle antioxidant performance of beef cattle.(Experimental design)A total of 80 healthy western beef cattle with body weight of(390.47±28.31)kg were divided into 4 groups with 20 cows per group and 1 replicate per cow.The experimental diets were supplemented with 0,150,300 and 600 mg/kgα-LA in the basal diet,respectively.The test was measured for 100 days,including a one-week adaptation cycle.The results showed that,compared with the control group,FBW in 300 and 600 mg/kgα-LA groups was significantly increased by 2.59%and 1.80%,respectively(P<0.05).ADG of beef cattle in 150-600 mg/kgα-LA group was significantly increased by 7.79%,9.37%and 7.22%,respectively(P>0.05);F/G of beef cattle in the 150~600 mg/kgα-LA group was significantly decreased by 5.94%,7.57%and 6.18%,respectively(P>0.05).In addition,α-LA had no significant effect on beef cattle ADFI(P>0.05).Compared with control group,beef pH and redness of beef in 150-600 mg/kgα-LA group were significantly increased(P<0.05);Beef yellowness in 150-600 mg/kgα-LA group was significantly decreased(P<0.05);Beef shear strength of beef cattle in 150-600 mg/kgα-LA group was significantly decreased(P<0.05);In addition,α-LA had no significant effects on the brightness,drip loss and cooking loss of beef(P>0.05).Keap1 gene expression in longissimus dorsi muscle of beef cattle in 300 and 600 mg/kgα-LA groups was significantly decreased(P<0.05);SOD gene expression in longissimus dorsi muscle of beef cattle in 150-600 mg/kgα-LA group was significantly increased(P<0.05);Keap1 gene expression in hind leg muscle of beef cattle in 150-600 mg/kgα-LA group was significantly decreased(P<0.05);The expression of Nrf-2 gene in hind leg muscle of beef cattle in 300 and 600 mg/kgα-LA groups was significantly increased(P<0.05);SOD gene expression in hind leg muscle of beef cattle in 600 mg/kgα-LA group was significantly increased(P<0.05).Conclusion:α-LA supplementation can promote the growth of beef cattle.In addition,α-LA can improve meat quality by regulating the expression of antioxidant genes in beef.