蓝靛果多酚调控肠道健康缓解小鼠低温运动疲劳研究

Effect of Lonicera caerulea Berry Polyphenols on Regulate Intestinal Health and Relief Exercise Fatigue of Mice in Low Temperature

长期高强度低温体育运动会造成机体疲劳损伤,降低运动员的成绩。该研究评价了蓝靛果多酚(Lonicera caerulea berry polyphenols,LCBP)对小鼠低温(-5℃)跑步机运动疲劳的影响,以VC为阳性对照,测定了结肠氧化、产物代谢(乳酸(Lactic acid,LA)、血尿素氮(Blood urea nitrogen,BUN)、血红蛋白(Hemoglobin,Hb)、肌酸激酶(Creatine kinase,CK)、乳酸脱氢酶(Lactate dehydrogenase,LDH)等生化指标。结肠苏木精-伊红(Hematoxylin-eosin,H&E)染色检测结肠病理,气相色谱-质谱检测结肠内容物短链脂肪酸靶向代谢和16S rRNA高通量测序小鼠肠道菌群。结果表明,与模型组相比,膳食增补LCBP极显著延长了小鼠跑步机运动的力竭时间27.4%(P<0.01);血液中Hb水平升高,LA、BUN和CK水平极显著降低(P<0.01),膳食补充LCBP(250 mg/kg bw)可极显著降低低温运动诱导的小鼠结肠组织丙二醛水平(P<0.01),增加抗氧化酶活力,提升总抗氧化水平(P<0.01);促进短链脂肪酸中乙酸、丙酸、丁酸、戊酸和己酸的生成(P<0.01),极显著改善肠道菌群,增加普雷沃氏菌属(Prevotella)、毛螺菌属(Lachnospira)和双歧杆菌属(Bifidobacterium)等有益菌属相对丰度和种类,促进肠道健康。总之,膳食增补LCBP可减少氧化应激、代谢产物积累,平衡肠道菌群稳态缓解小鼠低温运动疲劳。

Long-term high-intensity sports would cause fatigue damage and reduce the performance of athletes,especially low-temperature sports.This study evaluated the effects of Lonicera caerulea berry polyphenols(LCBP)on treadmill fatigue in mice at low temperature(-5℃).With VC as positive control,colon oxidation,product metabolism(lactic acid(LA),blood urea nitrogen(BUN)),hemoglobin(Hb),creatine kinase(CK),lactate dehydrogenase(LDH)and other biochemical parameters were determined.Colon pathology was detected by hematoxylin-eosin(H&E)staining.The short-chain fatty acid targeted metabolism of colon contents was detected by gas chromatography-tandem mass spectrometry(GC-MS),and intestinal flora was detected by 16S rRNA high-throughput sequencing.The results showed that compared with model group,dietary supplementation with LCBP significantly extended the exhaustion time of treadmill exercise by 27.4%(P<0.01).Hb levels in blood were increased,and LA,BUN and CK levels were significantly decreased(P<0.01).Dietary supplementation with LCBP(250 mg/kg bw)decreased the malondialdehyde(MDA)level in colonic tissues induced by low temperature exercise significantly(P<0.01),meanwhile,increased the activity of antioxidant enzymes,and the total antioxidant level(P<0.01).LCBP promoted the formation of acetic acid,propionic acid,butyric acid,valeric acid and caproic acid in short-chain fatty acids(P<0.01),improved intestinal flora significantly.And increased the abundance and species of beneficial bacteria such as Prevotella,Lachnospira and Bifidobacterium which promoted intestinal health.In conclusion,dietary supplementation of LCBP reduce oxidative stress,metabolite accumulation,balance intestinal flora homeostasis and relieve fatigue during low-temperature exercise in mice.