辽东楤木总皂苷联合酵母硒对C57小鼠的降血糖作用

Effects of total saponins of Aralia elata combined with yeast selenium on reducing the blood sugar in C57 mice

[目的]探究辽东楤木总皂苷联合酵母硒对2型糖尿病小鼠血糖的影响,为研制治疗糖尿病的新药物提供参考。[方法]选用60只C57小鼠,10只为对照组(A组),其余采用30 mg/kg链脲佐菌素建立糖尿病模型作为试验组。建模成功后随机分成B、C、D、E、F组,即模型组、二甲双胍组、辽东楤木总皂苷+酵母硒(120 mg/kg+1.5 mg/kg)组、辽东楤木总皂苷+酵母硒(120 mg/kg+1.0 mg/kg)组、辽东楤木总皂苷+酵母硒(120 mg/kg+0.5 mg/kg)组,每组各10只,连续治疗4周,采用血糖仪、全自动糖化血红蛋白分析仪、全自动生化分析仪对C57小鼠尾静脉血液中空腹血糖、糖化血红蛋白、血脂、口服葡萄糖耐量进行检测。[结果]与B组相比,各试验组均有不同程度的变化,C组、D组优于其他各组,且二组之间差异不显著。D组FBG降低了4.86 mmol/L,HbA1c水平下降了3.87%,TC、TG和LDL水平分别下降了1.18、0.02、1.72 mmol/L,而HDL显著升高0.42 mmol/L。口服糖耐量检测2 h恢复至餐前水平。[结论]120 mg/kg辽东楤木总皂苷联合1.5 mg/kg酵母硒降血糖效果最佳。

[Objectives]The effects of total saponins of Aralia elata combined with yeast selenium on reducing the blood sugar in type 2 diabetic(C57)mice were studied to provide a reference for the development of new drugs for the treatment of diabetes.[Methods]10 of 60 type 2 diabetic(C57)mice selected were used as the control group(Group A),and the rest were used to establish diabetes model with 30 mg/kg streptozotocin as the experimental group.The mice were randomly divided into groups B,C,D,E,and F after successful modeling,namely the model group,metformin group,total saponins of Aralia elata+yeast selenium(120 mg/kg+1.5 mg/kg)group,total saponins of Aralia elata+yeast selenium(120 mg/kg+1.0 mg/kg)group and total saponins of Aralia elata+yeast selenium(120 mg/kg+0.5 mg/kg)group,with 10 C57 mice in each group,and treated continuously for 4 weeks.The blood glucose meter,fully automatic glycated hemoglobin analyzer,and fully automatic biochemical analyzer were used to measure the content of fasting blood glucose(FBG),glycated hemoglobin,blood lipids,and oral glucose tolerance in the blood of tail vein in C57 mice.[Results]Each experimental group had varying degrees of changes compared with group B,with group C and group D outperforming the other groups,and the difference between the two groups was not significant.In group D,the content of FBG,HbA1c,TC,TG and LDL decreased by 4.86 mmol/L,3.87%,1.18,0.02,1.72 mmol/L,while the content of HDL significantly increased by 0.42 mmol/L.The Oral glucose tolerance testing restored to pre-meal levels within 2 hours.[Conclusions]The combination of 120 mg/kg total saponins of Aralia elata and 1.5 mg/kg yeast selenium had the best effect.