WIP1在口腔鳞状细胞癌中的表达及其与MYLK3的相关性研究

Expression of WIP1 and its correlation with MYLK3 in oral squamous cell carcinoma

目的探讨野生型P53诱导的磷酸酶1(Wild-type P53-induced phosphatase1,WIP1)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及其与肌球蛋白轻链激酶3(myosin light chain kinase 3,MYLK3)的相关性。方法采用免疫组化ABC法检测WIP1及MYLK3在65对OSCC及癌旁正常口腔黏膜中的表达,并通过肿瘤基因组图谱(the Cancer Genome Atlas,TCGA)数据库对WIP1在OSCC中的表达加以验证。分析WIP1的表达与患者年龄、性别、肿瘤分化程度及淋巴结转移等临床病理学指标的关系及其与MYLK3的相关性。结果WIP1在OSCC中的阳性表达率明显高于癌旁正常口腔黏膜中的阳性表达率(47.7%vs 24.6%,P<0.05);对TCGA数据库32对OSCC及癌旁正常口腔黏膜组织分析显示,WIP1与MYLK3在OSCC的阳性表达率均显著高于二者在癌旁正常口腔黏膜中的阳性表达率;WIP1在低分化组的阳性表达率明显高于其在高分化组的阳性表达率(86.7%vs 36.0%,P<0.05);WIP1在伴淋巴结转移组的阳性表达率明显高于其在无淋巴结转移组的阳性表达率(90.0%vs 40.0%,P<0.05);WIP1与患者性别、年龄、部位、肿瘤大小无相关性(P>0.05);WIP1与MYLK3在OSCC中的表达呈正相关(r=0.531,P<0.001)。结论WIP1在OSCC的发生、发展、分化及转移过程中可能发挥重要作用,有望成为判断OSCC发生、发展及预测淋巴结转移的新的潜在生物学标志物,其作用机制可能与MYLK3在OSCC中的正协同作用有关。

Objective To investigate the role of wild-type P53-induced phosphatase1(WIP1)and its correlation with myosin light chain kinase 3(MYLK3)in oral squamous cell carcinoma(OSCC).Methods The expression of WIP1 and MYLK3 in 65 pairs of OSCC and adjacent normal oral mucosa tissues was detected by immunohistochemical ABC method.The expression of WIP1 and MYLK3 in OSCC was verified by the Cancer Genome Atlas(TCGA)database.The relationship between the expression of WIP1 and the clinicopathological parameters such as age,gender,tumor differentiation and lymph node metastasis and the correlation between WIP1 expression and MYLK3 were analyzed.Results The positive expression rate of WIP1 in OSCC was significantly higher than that in adjacent normal oral mucosa(47.7%vs 24.6%,P<0.05).The TCGA database analysis of 32 pairs of OSCC and adjacent normal oral mucosa tissues showed that the positive expression rate of WIP1 and MYLK3 in OSCC were significantly higher than those in adjacent normal oral mucosa tissues.The positive expression rate of WIP1 in the poorly differentiated group was significantly higher than that in the well differentiated group(86.7%vs 36.0%,P<0.05).The positive expression rate of WIP1 in the group with lymph node metastasis was significantly higher than that in the group without lymph node metastasis(90.0%vs 40.0%,P<0.05).There was no correlation between WIP1 and gender,age,tumor location and tumor size(P>0.05);There was a positive correlation between the expression of WIP1 and MYLK3 in OSCC(r=0.531,P<0.001).Conclusion WIP1 may play an important role in the tumorigenesis,development,differentiation and metastasis of OSCC,and may be a new potential biomarker for the occurrence,development and lymph node metastasis of OSCC.The mechanism of WIP1 may be related to the positive synergistic effect of MYLK3 in OSCC.