比较3种微量肉汤稀释法检测念珠菌对氟康唑的敏感度

Comparison of three broth microdilution methods for the detection of fluconazole susceptibility of Candida

目的比较3种肉汤稀释药敏检测方法检测念珠菌对氟康唑敏感度的检测能力。方法收集河北医科大学第二医院临床分离的念珠菌84株,采取经典微量肉汤稀释法、ATB FUNGUS 3方法和Yeast One真菌药敏板方法检测念珠菌对氟康唑的敏感度。结果84株念珠菌中,白念珠菌26株,热带念珠菌34株,光滑念珠菌22株以及近平滑念珠菌2株。以经典微量肉汤稀释法为参考方法,ATB FUNGUS 3方法的敏感度为87.5%,特异度为100.0%;基本一致性为95.2%,分类一致性为92.9%;假耐药率为7.1%,假敏感率为0。Yeast One方法的敏感度为100.0%,特异度为94.4%;基本一致性和分类一致性均为97.6%,假敏感率和假耐药率均为0。结论Yeast One真菌药敏板方法检测氟康唑对念珠菌活性优于ATB FUNGUS 3方法。

Objective To compare three broth dilution methods in drug sensitivity detection for detecting the sensitivity of Candida to fluconazole.Methods A total of 84 clinical isolates of Candida were collected in the Second Hospital of Hebei Medical University,and the sensitivity of Candida to fluconazole was detected by classical broth microdilution method,ATB FUNGUS 3 and Sensititre Yeast One method.Results Among the 84 strains of Candida,there were 26 isolates of Candida albicans,34 ioslates of Candida tropicalis,22 isolates of Candida glabrata and 2 isolates of Candida parapsilosi.The classical broth microdilution method was used as a reference method,the sensitivity of ATB FUNGUS method was 87.5%,with a specificity of 100.0%.The essential agreement was 95.2%,and the categorical agreement was 92.9%;The false resistance rate was 7.1%,while there was no false sensitivity rate.The sensitivity and specificity of Sensititre Yeast One method were 100.0%and 94.4%respectively.Both the essential agreement and the categorical agreement were 97.6%,and the false sensitivity rate and the false resistance rate were 0.Conclusion Sensititre Yeast One method to detect the effect of fluconazole on Candida activity is superior to that of ATB FUNGUS 3.