丁香酚对南方根结线虫活性测定方法的比较

Comparison of three bioassay methods of eugenol against Meloidogyne incognita

为了准确评价丁香酚(C_(10)H_(12)O_(2))对南方根结线虫Meloidogyne incognita的毒杀效果,本研究采用水琼脂平板熏蒸法、土壤熏蒸法和浸虫法分别测定了丁香酚对南方根结线虫2龄幼虫(J_(2))和卵的毒力,比较3种测定方法的测定效果。结果表明,水琼脂平板熏蒸法、土壤熏蒸法和浸虫法测得的丁香酚对2龄幼虫的LC_(50)分别为123.5、140.2μL/L和309.7μL/L;丁香酚浓度为50μL/L时,水琼脂平板熏蒸法测得的卵孵化抑制率高达97.10%,而土壤熏蒸法和浸虫法测得的卵孵化抑制率仅分别为32.79%、11.50%;同时,水琼脂平板可作为线虫或卵的可视化载体,便于在体视显微镜下观察拍照。综上,水琼脂平板熏蒸法具有药效发挥更充分、可视化、操作简便等优点,该方法更适用于熏蒸类化合物的杀线虫生物测定。

In order to accurately evaluate the toxicity of eugenol(C_(10)H_(12)O_(2))on Meloidogyne incognita,the second-stage juveniles(J_(2))and eggs of M.incognita were treated with eugenol by water agar plate fumigation,soil fumigation,and immersion,respectively.The results showed that the LC_(50)value of eugenol by water agar plate fumigation was 123.5μL/L,while those by soil fumigation and immersion were 140.2μL/L and 309.7μL/L,respectively.The inhibition rate of egg hatching by 50μL/L eugenol with water agar plate fumigation was 97.10%,while those by soil fumigation and immersion method were only 32.79%and 11.50%,respectively.Meanwhile,the water agar plate could be used as a visual carrier of J_(2)or eggs under stereomicroscopy for easy observation.In summary,the water agar plate fumigation has the advantages of fully exerting virulence effect of eugenol,visualization,and simple operation.It is more suitable for the nematode bioassay of fumigation compounds.