基于16S rRNA基因测序分析食管鳞癌患者食管菌群分布特征

Distribution of Esophageal Microbiota in Patients with Esophageal Squamous Cell Carcinoma Analyzed Based on 16S rRNA Gene Sequencing

目的描述食管鳞癌(esophageal squamous cell carcinoma,ESCC)患者癌组织和邻近癌旁组织食管菌群组成和结构特点,筛选两组差异性菌群。方法收集安阳市肿瘤医院48例经病理学确诊的原发性ESCC的癌组织和邻近癌旁组织,对16S rRNA基因的5个变异区(V2、V3、V5、V6、V8)进行测序,采用Wilcoxon秩和检验比较两组菌群Alpha多样性及相对丰度差异。结果与癌旁对照组相比,ESCC癌组织Alpha多样性升高。厚壁菌门、拟杆菌门、变形菌门和梭杆菌门是ESCC组及癌旁对照组的主要菌门。和癌旁对照组相比,ESCC组含有较高丰度的梭杆菌门及螺旋菌门,较低丰度的放线菌门和蓝藻门(P<0.05)。属水平上,ESCC组梭杆菌属、嗜二氧化碳噬纤维菌属、卡氏菌属和密螺旋体属菌群丰度显著高于癌旁对照组,而葡萄球菌属和微单胞菌属丰度低于癌旁对照组(P<0.05);种水平上,ESCC组具核梭杆菌、牙周梭杆菌、生痰二氧化碳嗜纤维菌和牙龈卟啉单胞菌等丰度高于癌旁对照组,而异乳链球菌和鞘氨醇单胞菌丰度低于癌旁对照组。结论ESCC患者肿瘤和邻近正常肿瘤组织样本的食管微生物组成存在显著差异。

Objective To describe the composition and structure of esophageal microbiota of tumor and non-tumor tissues in patients with esophageal squamous cell carcinoma(ESCC)and to screen the differences between the two groups.Methods The cancer tissues and adjacent tissues of 48 cases of primary ESCC diagnosed by pathology in Anyang Cancer Hospital were collected,and 5R 16S rRNA getne(V2,V3,V5,V6,V8)sequencing was performed.Wilcoxon rank sum test was used to compare Alpha diversity and relative abundance between the two groups.Results The Alpha diversity of ESCC cancer tissue was higher than that of non-tumor control group.Firmicutes,Bacteroidota,Proteobacteria and Fusobacteriota were the main phyla in ESCC and non-tumor control groups.At the phylum level,the ESCC group contained higher abundances of Fusobacteriota and Spirochaetes,and lower abundances of Actinobacteria and Cyanobacteria(P<0.05)compared with the non-tumor control group.At the genus level,the abundance of Fusobacterium,Capnocytophaga,Catonella and Treponema in ESCC group were significantly higher than that in non-tumor control group,while the abundance of Staphylococcus and Pseudomonas in ESCC group were lower than that in non-tumor control group(P<0.05).At the species level,the abundance of Fusobacterium nucleatum,Fusobacterium periodonticum,Capnocytophaga sputigena and Porphyromonas gingivalis in ESCC group were higher,while the abundance of Streptococcus dysgalactiae and Sphingomonas leidyi were lower than that in non-tumor control group.Conclusion There were significant differences in esophageal microbiota composition between tumor and adjacent normal tumor tissue samples from ESCC patients.