秸秆生物炭与生物菌剂协同处理对土壤中重金属和抗生素的影响

Effects of Straw Biochar and Microbial Agent on Heavy Metals and Antibiotics in Soil

以秸秆生物炭(S)和微生物菌剂(MA)为试材,采用高锰酸钾滴定、氨释放量、氯仿熏蒸-浸提法、PCR方法,研究了秸秆生物炭与生物剂(SM)协同处理对土壤的性质、酶活性、微生物量的影响,以及对铅(Pb)、镉(Cd)及其抗性基因(MRGs)、四环素(T)和抗生素抗性基因(ARGs)去除,以期为实现以废治废、优化资源的研究提供参考依据。结果表明:施用SM后,土壤的总有机碳(TOC)、总氮(TN)、总磷(TP)、速效磷(AP)得到有效的改善。过氧化氢酶(CAT)、脲酶(URE)、蔗糖酶(SUC)的活性分别比对照(CK)提高了36.00%、45.00%、53.00%。微生物碳(MBC)和微生物氮(MBN)、微生物磷(MBP)也得到相应的变化。Cd-Pb-SM组与Cd-Pb组相比,Cd、Pb的修复率分别为35.49%和20.09%;Cd-Pb-T-SM组与Cd-Pb-T组相比,Cd、Pb修复率分别为23.69%和25.57%。Cd-Pb-SM组与Cd-Pb组相比,cad D、pbrT的修复率分别为31.94%和30.11%;Cd-Pb-T-SM组与Cd-Pb-T组相比,cad D、pbrT修复率分别为29.19%和35.74%。T-SM、Cd-T-SM、Pb-T-SM、Pb-Cd-T-SM组与对应的T、Cd-T、Pb-T、Pb-Cd-T组相比,T的修复率分别为23.83%、24.03%、10.85%和19.08%;Cd-Pb-T-SM组与Cd-Pb-T组相比,tet A、tet M分别减少30.32%和31.71%。综上,秸秆生物炭协同生物菌剂可有效地改善土壤的结构和循环,促进土壤的酶活性,对重金属、抗生素的单一和多重污染均产生良好的消减作用。

Taking the straw biochar(S)and microbial agents(MA)as the test material,the effects of SM synergistic treatment on soil properties,enzyme activities and microbial biomass were studied.Meantime,the removal of Pb,Cd,MRGs,antibiotic and ARGs were detected by titration,chloroform fumigation-extraction and PCR.Through the above researches,the foundation of realizing waste treatment and optimizing resources would been laid.The results showd that compared with CK,the TOC,TN,TP and AP in soil were significantly improved with SM.The activities of catalase,urease and invertase in soil with SM were increased by 36.00%,45.00%and 53.00%,respectively.MBC,MBN and MBP in siol were also increased.In Cd-Pb-SM and Cd-Pb-T-SM groups,the residual of Cd and Pb were reduced to 35.49%and 20.09%,respectively.While the repair rates of Cd and Pb were 23.69%and 25.57%in Cd-Pb-T-SM and Cd-Pb-T group,respectively.Compared with Cd-Pb group,the cad D and pbrT of Cd-Pb-SM group were decreased by 31.94%and 30.11%,respectively.While those of Cd-Pb-T-SM group were decreased by 29.19%and 35.74%,respectively.Compared T,Cd-T,Pb-T,Pb-Cd-T groups with T-SM,Cd-T-SM,Pb-T-SM,Pb-Cd-T-SM,Pb-Cd-T-SM groups,the residues of tetracycline were increased towere 23.83%,24.03%,10.85%,19.08%,respectively.Compared Cd-Pb-T-SM with Cd-Pb-T-SM group,tet A and tet M were decreased to 30.32%and 31.71%,respectively.In conclusion,all results had showed that the straw biochar combined with microbial agent could effectively improved soil structure and circulation,promoted soil enzyme activity.Meamtime,these also had been effected on multi-pollution of heavy metals and antibiotics.