多功能海藻多糖复合膜的制备及其对UVB诱导的光老化的保护作用

Preparation of a multifunctional seaweed polysaccharide-based composite film and its protective effects against UVB-induced photoaging

长期暴露在紫外线B(UVB)的照射下会导致皮肤光老化。海藻多糖是一类结构复杂的海洋酸性多糖,具有抗氧化、抗炎以及免疫调节等多种生物活性。其寡糖因分子量低、水溶性好等特点,具有更好的生物活性和应用潜力。本文以硫酸化半乳岩藻聚糖(DF)和褐藻胶寡糖(AOS)为活性成分,κ-卡拉胶(κ-Car)为基质制备了多功能海藻多糖复合膜——FAC膜,通过理化性质表征以及体内实验探究其对UVB诱导的皮肤光老化的保护作用。理化测定结果表明, FAC膜具有良好的吸湿和保湿性,在不同pH环境下具有缓释DF的效果。FAC膜在去离子水中浸泡3 min后,具有较好的黏弹性。体内实验结果表明,FAC膜处理可提高皮肤含水量,减少UVB诱导引起的皮肤的表皮层和真皮层厚度的增加;提高皮肤过氧化氢酶(CAT)和超氧化物歧化酶(SOD)浓度;降低促炎症因子白介素-6(IL-6)和肿瘤坏死因子(TNF-α)的表达;提高胶原蛋白特征氨基酸酶羟脯氨酸(HyP)的浓度以及有效降低胶原蛋白降解酶基质——金属蛋白酶-3(MMP-3)的表达。这些结果表明, FAC膜具有保湿、缓释和便于使用等良好理化性质以及修复皮肤屏障、抗氧化、抗炎和抑制胶原蛋白降解的良好生物活性,可以作为预防和治疗皮肤光老化的制剂。

Long-term exposure to ultraviolet B(UVB)radiation causes photoaging of the skin.Seaweed-derived polysaccharides are a class of structurally complex,acidic,marine polysaccharides with various biological activities such as antioxidant,anti-inflammatory,and immunomodulatory effects.Their oligosaccharides have better bioac-tivity and application potential due to low molecular weight and good water solubility.In this paper,a multifunc-tional seaweed polysaccharide-based composite film,the FAC film,was prepared using sulfated galactofucan poly-saccharides(DF)and oligosaccharides sodium alginate(AOS)as active ingredients,andκ-carrageenan(κ-Car)as the matrix.Next,their protective effects against UVB-induced skin photoaging were investigated via physico-chemical property characterization and in vivo experiments.The results of the physicochemical measurements showed that the film had good hygroscopic and moisturizing properties and a slow-release DF effect in different pH environments.The film exhibited good viscoelasticity after soaking in deionized water for 3 mins.The results of the in vivo experiments revealed that treatment with FAC film enhanced the skin water percentage and reduced UVB-induced thickening of the epidermis and dermis of the skin;elevated skin catalase(CAT)and superoxide dismutase(SOD)concentrations;suppressed expression of the pro-inflammatory factors interleukin-6(IL-6)and tumor necrosis factor(TNF-α);increased levels of the collagen characteristic amino acid hydroxyproline(HyP);and effectively reduced the expression of the collagen degrading enzyme substrate,metalloproteinase-3(MMP-3).These results suggested that the FAC film has excellent physicochemical properties such as moisturization,slower release,and ease of application,as well as appreciable biological activities of skin barrier repair and antioxidant,anti-inflammatory,and collagen degradation inhibition effects.Thus,it could be utilized as an agent for the preven-tion and treatment of skin photoaging.