鼻咽癌组织SNHG15和LINC00261的表达及其临床意义

The expression and clinical significance of SNHG15 and LINC00261 in nasopharyngeal carcinoma t

目的:探讨鼻咽癌中小核仁RNA宿主基因15(SNHG15)和LINC00261的表达水平及其临床意义。方法:收集2018年1月至2020年2月本院93例行鼻咽癌根治性切除术患者的鼻咽癌组织和癌旁组织样本以及临床资料;培养鼻咽癌CNE-1细胞和正常鼻咽部上皮NP69细胞,分为空白对照组、shRNA-NC组(转染SNHG15对照)、SNHG15-shRNA组(转染SNHG15)、pcDNA-NC组(转染LINC00261对照)、LINC00261-pcDNA组(转染LINC00261);采用RT-qPCR检测SNHG15和LINC00261表达水平;采用CCK-8和克隆形成实验检测各组细胞的增殖情况;采用Pearson法分析组织中SNHG15和LINC00261表达水平的相关性;采用Kaplan-Meier法分析鼻咽癌组织SNHG15和LINC00261表达水平与患者预后的关系;采用多因素Cox回归分析影响鼻咽癌患者预后的相关因素。结果:鼻咽癌组织中SNHG15表达水平高于癌旁组织,LINC00261表达水平则低于癌旁组织;鼻咽癌组织中SNHG15和LINC00261表达水平呈负相关。CNE-1细胞中SNHG15表达水平高于NP69细胞,LINC00261表达水平则低于NP69细胞;SNHG15-shRNA组和LINC00261-pcDNA组CNE-1细胞克隆形成数量均低于空白对照组、shRNA-NC组及pcDNA-NC组,细胞增殖能力降低。不同SNHG15和LINC00261表达水平的鼻咽癌患者年龄、肿瘤直径及组织学类型差异无统计学意义,而SNHG15高表达组及LINC00261低表达组发生淋巴结转移、TNM分期为Ⅲ+Ⅳ期的患者所占比例显著高于SNHG15低表达组及LINC00261高表达组;SNHG15低表达患者3年生存率(84.78%)显著高于SNHG15高表达患者(65.95%),LINC00261低表达患者3年生存率(63.04%)显著低于LINC00261高表达患者(87.23%)。结论:鼻咽癌组织中SNHG15和LINC00261表达与患者临床病理特征及预后密切相关,淋巴结转移、TNM分期及SNHG15是鼻咽癌患者死亡的危险因素,而LINC00261是鼻咽癌患者死亡的保护因素。

Objective:To explore the expression of small nucleolar RNA host genes 15(SNHG15)and LINC00261 in nasopharyngeal carcinoma tissues and their clinical significance.Methods:Tumor tissue and adjacent tissue samples of 93 cases of nasopharyngeal carcinoma undergoing radical resection in the Department of Otolaryngology of our hospital from January 2018 to February 2020 were collected,as the well as clinical data of patients;The cultured nasopharyngeal carcinoma CNE-1 cells and normal nasopharyngeal epithelial NP69 cells were divided into blank control group,shRNA-NC group(transfected with SNHG15 control),SNHG15 shRNA group(transfected with SNHG15),pcDNA-NC group(transfected with LINC00261 control),and LINC00261 pcDNA group(transfected with LINC00261);RT-qPCR was used to detect the expression of SNHG15 and LINC00261 in CNE-1 cells.CKK-8 method and clone formation assay were used to detect the proliferation of cells in each group.Pearson method was applied to analyze the correlation between the expression of SNHG15 and L INC00261 in nasopharyngeal carcinoma tissues.Kaplan-Meier method was applied to analyze the relationship between the expression of SNHG15 and LINC00261 in nasopharyngeal carcinoma tissues and prognosis.Multivariate Cox regression analysis was applied to investigate the factors affecting the prognosis of nasopharyngeal carcinoma patients.Results:The expression of SNHG15 in nasopharyngeal carcinoma tissues was higher than that in adjacent tissues,while the expression of LINC00261 was lower than that in adjacent tissues.The expression of SNHG15 and LINC00261 in nasopharyngeal carcinoma tissue were negatively correlated.The expression of SNHG15 in pharyngeal cancer cells was higher than that in normal nasopharyngeal epithelial cells,while the expression of LINC00261 was lower than that in normal nasopharyngeal epithelial cells.The number of cell clones formed in the SNHG15-shRNA group and LINC00261-pcDNA group was lower than that in the control group,shRNA-NC group,and pcDNA-NC group,indicating a decrease in cell proliferation ability,and there was no statistically significant difference in age,tumor diameter,and histological type among nasopharyngeal carcinoma patients with different levels of SNHG15 and LINC00261 expression,while the proportions of patients with lymph node metastasis and TNM stageⅢ+Ⅳin the SNHG15 high expression group and LINC00261 low expression group were higher than those in the SNHG15 low expression group and LINC00261 high expression group.The three-year survival rate(84.78%)of the SNHG15 low expression patients was higher than that of the SNHG15 high expression patients(65.95%),while the threeyear survival rate(63.04%)of the LINC00261 low expression patients was lower than that of the LINC00261 high expression patients(87.23%).The lymph node metastasis,TNM staging,and SNHG15 were risk factors for death in nasopharyngeal carcinoma patients,while LINC00261 was a protective factor for such patients.Conclusion:The expression of SNHG15 and LINC00261 in nasopharyngeal carcinoma tissues are closely related to the clinical pathological characteristics and prognosis of patients.