优化HPLC法测定不同基质中环丙氨嗪残留量

Optimization of HPLC Method for the Determination of Cyromazine Residues in Different Matrices

在《动物性食品中环丙氨嗪残留量的测定高效液相色谱法》(GB 31658.12—2021)的基础上,优化前处理方法,使用1%氨水乙腈溶液提取,正己烷去油脂,QuEChERS法净化,在不同基质中进行加标回收率验证。结果表明,该方法的回收率在78.26%~82.82%,精密度相对标准偏差为3.05%~7.71%;环丙氨嗪质量浓度在0.05~2.00 mg·L^(-1)范围内线性关系较好,R^(2)=0.999 9;其检出限的信噪比大于3,定量限的信噪比大于10,能满足检测要求。此方法操作起来简单快捷,适用于环丙氨嗪残留量的大批量检测。

Based on GB 31658.12—2021,the pre-treatment method is optimized,the samples are extracted with 1%ammonia in acetonitrile solution,degreased with hexane,and purified by QuEChERS method.The recoveries are verified in different matrices.The results show that the recovery rate of the method is 78.26%to 82.82%,and the precision is 3.05%to 7.71%;the linear relationship is good in the range of 0.05~2.00 mg·L^(-1),R^(2)=0.9999;the limited signal-noise ratio is greater than 3,and the signal-noise ratio of quantitative limit is greater than 10,which can meet the test requirements.This method is simple and fast to operate,and is suitable for the detection of cyromazine residue in large quantities.