高效液相色谱法测定橙汁中山梨酸含量的不确定度评定

Evaluation of Uncertainty in Determination of Sorbic Acid in Orange Juice by High Performance Liquid Chromatography

目的:评定高效液相色谱法测定橙汁中山梨酸含量的不确定度,确保检测结果的准确性和可靠性。方法:采用《食品安全国家标准食品中苯甲酸、山梨酸和糖精钠的测定》(GB 5009.28—2016)中第一法液相色谱法对2个考核样品和内控样品中山梨酸含量进行测定,建立数学模型,计算合成标准不确定度和扩展不确定度。结果:考核样品1中山梨酸含量为(0.03423±0.00144)g·kg^(-1)(k=2),考核样品2中山梨酸的含量为(0.05743±0.00172)g·kg^(-1)(k=2)。结论:该测定结果的不确定度主要来源于高效液相色谱仪的重复性测定、标准曲线拟合、山梨酸标准溶液浓度,因此在样品测定时要对这些因素加以控制,以提高检测结果的准确性。

Objective:The uncertainty of determination of sorbic acid in orange juice by HPLC was evaluated to ensure the accuracy and reliability of the test results.Method:The content of sorbic acid in two blind samples and quality control samples was determined by liquid chromatography,the first method in GB 5009.28—2016,and a mathematical model was established to calculate the synthesis standard uncertainty and extended uncertainty.Result:The sorbic acid content in sample 1 was(0.03423±0.00144)g·kg^(-1)(k=2),and the sorbic acid content in sample 2 was(0.05743±0.00172)g·kg^(-1)(k=2).Conclusion:The uncertainty in the determination result is mainly derived from the repeatability determination of high performance liquid chromatography,standard curve fitting and sorbic acid standard solution concentration.Therefore,these factors should be controlled during the sample determination to improve the accuracy of the determination results.