白茅根提取物抑菌抗炎活性在口腔护理品中研究与分析

Study and analysis of antibacterial and anti-inflammatory activity of extracts of Rhizoma imperatae in oral care products

采用体外评价方法对白茅根提取物在口腔护理品中的抑菌抗炎活性进行研究。采用水、50%乙醇、乙酸乙酯、丙酮、石油醚作为溶剂提取白茅根提取物,将其加入到二氧化硅体系牙膏中,通过“划割牙龈+钢丝结扎+滴入牙周炎患者细菌培养物+高糖黏性食物饲养”构建20只5周龄SD大鼠牙周炎模型,测定白茅根提取物对大鼠牙周炎模型中的牙周致病菌[伴放线杆菌(Aa)、牙龈卟林单胞菌(Pg)、具核梭杆菌(Fn)、中间普菌(Pi)、硒赛类标菌(Tf)、变黑普菌(Pn)]、外周血、龈沟液内炎性因子[C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白介素-6(IL-6)、前列腺素E_(2)(PGE_(2))]及牙周健康指标[菌斑指数(PLI)、探诊深度(PD)、出血指数(BI)]的影响,采用小白鼠急性经口毒性实验验证白茅根提取物应用于口腔护理品中的安全性。结果显示,白茅根提取物能明显减少Aa、Pg、Fn、Pi、Tf、Pn,降低CRP、TNF-α、IL-1β、IL-6、PGE_(2)表达,具有抑制牙周致病菌活性,抵抗牙周炎症反应作用,能改善牙周健康,且安全性可靠。

In vitro evaluation method was used to study the antibacterial and anti-inflammatory activity of extracts of Rhizoma imperataein in oral care products.Water,50%ethanol,ethyl acetate,acetone and petroleum ether were used as solvents to extract Rhizoma imperatae,which was added to the toothpaste of silica system.The periodontitis model of 205-week-old SD rats was established by“cutting the gum+wire ligation+drop into the bacterial culture of periodontitis patients+feeding with high sugar sticky food”.The effects of extract from Rhizoma imperatae on periodontal pathogens[Actinobacteria(Aa),Porphyromonas gingivalis(Pg),Fusobacterium nucleatum(Fn),Puccinia intermedia(Pi),Selenium class standard bacteria(Tf),Puccinia melanogenes(Pn)],peripheral blood,inflammatory factors in gingival crevicular fluid[C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),prostaglandin E_(2)(PGE_(2))],and periodontal health indicators[Plaque index(PLI),depth of exploration(PD),bleeding index(BI)]of the rat periodontitis model were determined,and the acute oral toxicity experiment in mice was used to verify the safety of the extracts of Rhizoma imperatae used in oral care products.The results show that the extracts of Rhizoma imperatae can significantly reduce Aa,Pg,Fn,Pi,Tf,Pn,and reduce the expression of CRP,TNF-α,IL-1β,IL-6,and PGE_(2).It has the activity of inhibiting periodontal pathogens,resisting periodontal inflammation,improving periodontal health,and is safe and reliable.