湖南和云南地区禽蛋、禽畜肉沙门氏菌污染的情况分析

Analysis of the pollution of poultry eggs、poultry and animal meat in Hunan and Yunnan regions

对随机抽取的湖南、云南地区的禽蛋、禽畜肉样品中沙门氏菌污染的情况进行调查分析。将所选取的样品先经过增菌、选择性平板分离培养,再经过生化鉴定、微生物全自动鉴定仪鉴定,荧光定量PCR鉴定和血清分型鉴定,基质辅助激光解析电离飞行时间质谱鉴定。检测分析鉴定结果发现,所选取的101批次禽蛋、禽畜肉样品总体受肠炎沙门氏菌、鼠伤寒沙门氏菌污染比例分别为35.64%,8.91%;其中禽蛋受肠炎沙门氏菌污染的比例为25.00%,受鼠伤寒沙门氏菌污染的比例为33.33%;禽畜肉受肠炎沙门氏菌污染的比例为37.08%,受鼠伤寒沙门氏菌污染的比例为5.62%。禽蛋总体受肠炎沙门氏菌和鼠伤寒沙门氏菌污染率为58.33%,高于禽畜肉总体的污染率42.70%。调查结果显示,禽蛋和禽畜肉受沙门氏菌污染情况不容乐观,在屠宰、生产加工、包装储运、食用过程中仍需要引起注意。

Salmonella contamination in poultry eggs and meat samples from Hunan and Yunnan were investigated and analyzed.The selected samples were first cultured by bacteria enhancement and selective plate isolation,then identified by biochemical identification,automatic microbial identification instrument,fluorescence quantitative PCR identification,serotyping identification,matrix assisted laser resolution ionization time-of-flight mass spectrometry identification.The results showed that the contamination rates of Salmonella enteritidis and Salmonella typhimurium in 101 batches of eggs and meat samples were 35.64%and 8.91%,respectively.The proportion of eggs contaminated by Salmonella enteritidis was 25.00%,and the proportion contaminated by Salmonella typhimurium was 33.33%.The proportion of meat contaminated with Salmonella enteritidis was 37.08%and that with Salmonella typhimurium was 5.62%.The contamination rate of poultry eggs with Salmonella enteritidis and Salmonella typhimurium was 58.33%,which was higher than that of poultry meat with 42.70%.The investigation results show that the situation of salmonella contamination in poultry eggs and poultry meat is not optimistic,and it still needs attention in the process of slaughter,production and processing,packaging,storage and transportation,and consumption.