超敏蛋白与壳寡糖对微咸水灌溉下番茄幼苗的影响

Effects of Hypersensitive Protein and Chitosan Oligosaccharide on the Growth,Photosynthesis,and Antioxidant Capacity of Tomato Seedlings under Brackish Water Irrigation

为研究微咸水育苗中盐害的缓解方法,合理利用超敏蛋白(HP)、壳寡糖(COS)制剂,以番茄品种玉林达为试材,于温室进行穴盘育苗,设置浸种、叶面喷施、灌溉方式共8个处理:CK1为全阶段淡水;CK2为全阶段微咸水(可溶性盐质量浓度为2.5 g/L);A1为淡水+500倍液HP+微咸水;A2为淡水+800倍液HP+微咸水;B1为200倍液HP+800倍液HP+微咸水;B2为400倍液HP+500倍液HP+微咸水;C1为200倍液HP+800倍液COS+微咸水;C2为400倍液HP+500倍液COS+微咸水,筛选超敏蛋白与壳寡糖制剂的最适处理浓度和方法。结果表明:在微咸水灌溉下,播前采用淡水或HP浸种,苗期叶面喷施COS或HP制剂可以显著促进番茄幼苗的生长发育。相比于CK2,A2的根系活力显著提高了277.15%;B2的净光合速率显著提高了34.96%,丙二醛含量、脯氨酸质量比显著降低;C2的气孔导度显著提高了8.33%;过氧化物酶活性和过氧化氢酶活性显著提升了221.13%和14.64%。主成分隶属函数分析表明,播前以400倍液HP浸种,苗期叶面喷施500倍液COS处理对缓解番茄幼苗盐胁迫的效果最优。推荐当地利用微咸水进行番茄育苗时采用该方案培育壮苗。

This study aims to explore methods for mitigating salt damage during seedling growth in brackish water and to rationally utilize hypersensitive protein(HP)and chitosan oligosaccharide(COS).The tomato variety Yulinda was used as the test material,employing three treatment stages:seed soaking,leaf spraying,and irrigation in a greenhouse seedling tray.Eight experimental treatments were established:CK1:full-stage freshwater;CK2:full-stage brackish water(EC=2.5 g/L);A1:freshwater+500 times HP+brackish water;A2:freshwater+800 times HP+brackish water;B1:200 times HP+800 times HP+brackish water;B2:400 times HP+500 times HP+brackish water;C1:200 times HP+800 times COS+brackish water;C2:400 times HP+500 times COS+brackish water.The objective was to determine the optimal concentrations and application methods for HP and COS.Results demonstrated that under brackish water irrigation,soaking seeds in freshwater or HP prior to sowing,and spraying COS or HP during the seedling stage significantly improved the growth and development of tomato seedlings.Compared to the brackish water control(CK2),the root activity in A2 increased significantly by 277.15%.The net photosynthetic rate in B2 increased significantly by 34.96%,while the contents of malondialdehyde(MDA)and proline significantly decreased.Stomatal conductance in C2 increased significantly by 8.33%,and peroxidase and catalase activities increased significantly by 221.13%and 14.64%,respectively.Principal component analysis indicated that soaking seeds in 400 times HP before sowing and spraying 500 times COS at the seedling stage provided the optimal method for alleviating salt stress in tomato seedlings.This approach is recommended for cultivating robust seedlings under brackish water conditions.