宣肺败毒颗粒增加T细胞数量减轻流感病毒肺炎的机制研究

The mechanism of Xuanfei Baidu Granules to treat influenza virus pneumonia by increasing T cells

[目的]确定宣肺败毒颗粒在低温高湿环境下,A/PR/8/34(H1N1)流感病毒肺炎小鼠模型的治疗效果及作用机制。[方法]将ICR小鼠置于具有低温高湿环境的人工气候箱中,滴鼻给予流感病毒(A/PR/8/34)建立流感病毒肺炎模型。造模后第5天对小鼠每日给予宣肺败毒颗粒灌胃5 d,观察记录小鼠的行为学评分、体质量、脏器指数、肺部病毒载量、肺部影像学及形态学变化,蛋白印迹法检测炎症关键蛋白NF-κB的表达变化,流式细胞术检测宣肺败毒颗粒对小鼠免疫应答的调节。[结果]与模型组相比,宣肺败毒颗粒给药后小鼠表现出活跃程度上升、大便性状正常、肺部病毒载量降低、小鼠肺部炎性渗出减少,p-NF-κB P65表达降低,CD4+和CD8+T细胞百分比增高。[结论]宣肺败毒颗粒增加T细胞数量,减轻流感病毒肺炎小鼠的炎症反应。

[Objective] To establish a influenza virus(A/PR/8/34) pneumonia model under low temperature and high humidity,and evaluate the efficacy and mechanism of Xuanfei Baidu Granules(XFBD) against influenza.[Methods] ICR mice were placed in an artificial climate box with low temperature and high humidity,and influenza virus(A/PR/8/34) was given intranasally.On the 5th day after modeling,mice were given XFBD by gavage for 5 d.The behavioral score,body weight,organ index,pulmonary viral load,pulmonary imaging and morphological changes were observed and recorded.The expression of NF-κB,a key inflammatory protein,was detected by Western blot,and the regulation of XFBD on the immune response of mice was detected by flow cytometry.[Results] Compared with the model group,after administration of XFBD,mice showed increased activity,normal stool characteristics,decreased lung viral load,decreased inflammatory exudation in mice's lungs,decreased expression of p-NF-κB P65,and increased percentages of CD4~+ and CD8~+T cells.[Conclusion] XFBD increased the amount of T cells,and inhibited the inflammation of the influenza virus pneumonia under cold and high humidity conditions.