沙葱多糖对小鼠卵清白蛋白的免疫佐剂作用

Immunoadjuvant Effects of Allium mongolicum Regel Polysaccharide on Ovalbumin in Mice

本试验旨在研究沙葱多糖(AMRP)对模型抗原卵清白蛋白(OVA)免疫小鼠的佐剂作用。选择40只6~8周龄、体重为(20±2)g的无特定病原体(SPF)级雌性昆明小鼠,随机分为5组,分别为空白对照组(BC组)、OVA免疫对照组(OVA组)、OVA+低剂量沙葱多糖组(LAMRP组)、OVA+中剂量沙葱多糖组(MAMRP组)和OVA+高剂量沙葱多糖组(HAMRP组),每组8个重复,每个重复1只。预试期7 d,正试期34 d。第1~4天和第16~19天,BC组和OVA组小鼠灌胃生理盐水,LAMRP组、MAMRP组和HAMRP组分别灌胃15、20和25 mg/kg BW沙葱多糖,灌胃量为0.2 mL;第5天和第20天,BC组小鼠腹部皮下注射0.1 mL/只生理盐水,其余各组小鼠注射100μg/只OVA溶液,二免后2周进行样品采集。结果表明:1)与BC组和OVA组相比,不同剂量沙葱多糖对试验各阶段小鼠体重和增重率均无显著影响(P>0.05)。2)与BC组和OVA组相比,各剂量沙葱多糖组小鼠胸腺指数均无显著差异(P>0.05);MAMRP组脾脏指数极显著提高(P<0.01),脾脏生发中心直径显著提高(P<0.05)。3)与BC组和OVA组相比,MAMRP组和HAMRP组小鼠血清白细胞介素-10(IL-10)含量极显著提高(P<0.01);与OVA组相比,LAMRP组和MAMRP组血清干扰素-γ(IFN-γ)含量显著提高(P<0.05);各组间血清白细胞介素-2(IL-2)和白细胞介素-4(IL-4)含量均无显著差异(P>0.05)。4)与BC组相比,MAMRP组小鼠血清OVA特异性抗体免疫球蛋白G(IgG)含量极显著提高(P<0.01)。综上所述,沙葱多糖可提高小鼠对抗原OVA的免疫能力,进而表现出一定的佐剂效用,可以作为一种有效的疫苗佐剂诱导机体免疫应答;本试验条件下,以灌胃20 mg/kg BW沙葱多糖时佐剂效果较佳。

The aim of this experiment was to study the adjuvant effects of Allium mongolicum Regel polysaccharide(AMRP)on mice immunized with model antigen ovalbumin(OVA).Forty specific pathogen free(SPF)female Kunming mice aged 6 to 8 weeks and weighing(20±2)g were randomly divided into 5 groups,which were blank control group(BC group),OVA immune control group(OVA group),OVA+low-dose AMRP group(LAMRP group),OVA+medium-dose AMRP group(MAMRP group)and OVA+high-dose AMRP group(HAMRP group),with 8 replicates per group and 1 replicate per replicate.The pre-trial period lasted for 7 days and the experimental period lasted for 34 days.During days 1 to 4 and days 16 to 19,the mice in BC group and OVA group were administrated with normal saline by oral gavage,and those in LAMRP group,MAMRP group and HAMRP group were administrated with 15,20 and 25 mg/kg BW AMRP by oral gavage,respectively,with an amount of 0.2 mL.On day 5 and day 20,the mice in BC group were injected subcutaneously with 0.1 mL normal saline every mouse,and those in the other groups were injected with 100μg OVA solution every mouse.Samples were collected for 2 weeks after second immunization.The results showed as follows:1)compared with BC group and OVA group,different doses of AMRP had no significant effects on body weight and weight gain rate of mice at each stage of the experiment(P>0.05).2)Compared with BC group and OVA group,there were no significant differences in thymus index of mice in each dose of AMRP group(P>0.05);the spleen index(P<0.01)and germinal center diameter in spleen(P<0.05)in MAMRP group were significantly increased.3)Compared with BC group and OVA group,the serum interleukin-10(IL-10)content of mice in MAMRP group and HAMRP group was extremely significantly increased(P<0.01);compared with OVA group,the serum interferon-γ(IFN-γ)content in LAMRP group and MAMRP group was significantly increased(P<0.05);there were no significant differences in serum contents of interleukin-2(IL-2)and interleukin-4(IL-4)among all groups(P>0.05).4)Compared with BC group,the OVA specific antibody immunoglobulin G(IgG)content in serum of mice in MAMRP group was extremely significantly increased(P<0.01).In conclusion,AMRP can improve the immune ability of mice against antigen OVA,and then show certain adjuvant effects,which can be used as an effective vaccine adjuvant to induce immune response;under the experimental conditions,the adjuvant effects are better when 20 mg/kg BW AMRP is administrated by oral gavage.