动物双歧杆菌乳亚种XLTG11对小鼠肠道的益生作用

Probiotic Effect of Bifidobacterium animalis subsp.lactis XLTG11 on the Intestinal Tract of Mice

该研究探讨了动物双歧杆菌乳亚种XLTG11对小鼠肠道的益生作用。将48只BALB/c小鼠适应性喂养7 d后,随机分成空白对照组、XLTG11低剂量组、XLTG11中剂量组和XLTG11高剂量组。其中受试组每只分别灌胃1.6×10^(6)、1.6×10^(7)和81.6×10^(8)CFU的动物双歧杆菌乳亚种XLTG11菌粉,空白对照组小鼠灌胃等体积的生理盐水。灌胃14 d后,测定小鼠体质量、肠道菌群、组织病理、肠道屏障、肠道通透性和短链脂肪酸含量。结果表明,与空白对照组相比,高剂量组小鼠体质量增加14.51%,小鼠肠道的菌群丰度和菌群结构发生改变,小鼠粪便中双歧杆菌和乳杆菌的数量分别增加29.32%和33.88%,产气荚膜梭菌的数量下降24.49%,并且低、中、高剂量组紧密连接蛋白和黏蛋白的表达量增加,小鼠血清中LPS和D-乳酸含量下降,表明动物双歧杆菌乳亚种XLTG11可以增强肠道屏障功能,降低肠道通透性。此外,小鼠盲肠中短链脂肪酸的含量分别升高13.66%、43.04%、48.58%。综上,该研究表明动物双歧杆菌乳亚种XLTG11能够对小鼠肠道产生健康益处,并对肠道菌群具有调节和改善作用。

The probiotic effects of Bifidobacterium animalis subsp.lactis XLTG11 on the intestinal tract of mice were investigated.After adaptive feeding for seven days,48 BALB/c mice were randomly divided into a blank control group,XLTG11 low dose group,XLTG11 middle dose group,and XLTG11 high dose group.The three subject groups had B.animalis 678 Lactis subspecies XLTG11 bacterial powder gavage,with doses of 1.6×10^(7) CFU/each,1.6×10^(8) CFU/each,and 1.6×10^(9) CFU/each,respectively.The gavage of the blank control group had an equal volume of saline.After 14 days of gavage,the body mass,intestinal microbiota,histopathology,intestinal barrier,intestinal permeability,and short-chain fatty acid content of mice were measured.The results indicated that,compared to the blank control group,the body mass of mice in the high-dose group increased by 14.51%.Changes occurred to the intestinal microbiota abundance and composition,with the numbers of Bifidobacterium and Lactobacillus in the feces of mice increasing by 29.32%and 33.88%,respectively.The quantity of Clostridium perfringens decreased by 24.49%,and the expression level of tight junction proteins and mucins increased in the low-,medium-,and high-dose groups.The levels of LPS and D-lactic acid in the sera of mice also decreased.These findings indicated that B.animalis subsp.lactis XLTG11 could enhance intestinal barrier function and reduce intestinal permeability.In addition,the levels of short-chain fatty acids in the cecum of mice in the three groups were 13.66%,43.04%,and 48.58%,respectively.In conclusion,this study demonstrated that B.animalis subsp.lactis XLTG11 could confer health benefits to the intestine in mice and had a regulatory and ameliorative effect on the intestinal microbiota.