急性心肌梗死并发肺部感染患者血清miR-93和miR-135a表达及意义

Expressions and significance of serum miR-93 and miR-135a in patients with acute myocardial infarction complicated by pulmonary infection

目的 探究miR-93和miR-135a在急性心肌梗死(AMI)并发肺部感染患者血清中的表达及意义。方法 收集2018年4月至2021年4月期间于孝感市中心医院就诊的AMI并发肺部感染患者107例为疾病组,另选取孝感市中心医院同期健康体检者107例为对照组。收集整理AMI并发肺部感染患者年龄、高血压、糖尿病等临床资料,随访2年。根据患者的随访情况又分为生存组和死亡组,比较两组的临床资料;采用qRT-PCR法检测血清miR-93和miR-135a表达;Pearson法分析疾病组患者血清miR-93和miR-135a水平的相关性;Logistic回归分析AMI并发肺部感染患者预后的影响因素。结果 疾病组患者血清miR-93表达水平高于对照组(P<0.05);miR-135a表达水平低于对照组(P<0.05);AMI并发肺部感染患者血清miR-93表达和miR-135a表达呈负相关(r=-0.532,P<0.001);与生存组相比,死亡组患者血清miR-93表达水平显著升高(P<0.05),miR-135a表达水平显著降低(P<0.05);miR-93和miR-135a均是AMI并发肺部感染患者预后的独立影响因素(P<0.05)。血清miR-93、miR-135a以及二者联合预测AMI并发肺部感染患者预后的曲线下面积(AUC)分别为0.835、0.887和0.955。结论 AMI并发肺部感染患者血清miR-93升高,miR-135a表达水平降低,两者可能是预测AMI并发肺部感染患者预后的有效指标。

Objective To discuss the expressions and significance of serum miR-93 and miR-135a in patients with acute myocardial infarction(AMI)complicated by pulmonary infection.Methods The patients with AMI complicated by pulmonary infection were chosen into disease group,and healthy controls were chosen into control group(each n=107)from Central Hospital of Xiaogan City from Apr.2018 to Apr.2021.The clinical materials,including age,hypertension and diabetes,were collected from the patients and they were followed up for 2 y.The patients were also divided,according to follow-up results,into survival group and death group,and clinical materials were compared between 2 groups.The expressions of miR-93 and miR-135a were detected by using qRT-PCR.The correlated between miR-93 level and miR-135a level were analyzed by using Pearson method.The factors affecting prognosis were analyzed by using Logistic regression analysis in AMI patients complicated by pulmonary infection.Results The expression of miR-93 was higher(P<0.05)and expression of miR-135a was lower(P<0.05)in disease group than those in control group.There was a negative correlated between miR-93 expression and miR-135a expression(r=-0.532,P<0.001)in AMI patients complicated by pulmonary infection.The expression of miR-93 increased significantly(P<0.05)and expression of miR-135a decreased significantly(P<0.05)in survival group compared with death group.The expressions of miR-93 and miR-135a were independent influence factors in AMI patients complicated by pulmonary infection(P<0.05).In predicting prognosis in AMI patients complicated by pulmonary infection,AUC of miR-93 was 0.835,AUC of miR-135a was 0.887,and AUC of miR-93 combined with miR-135a was 0.955.Conclusion The increased miR-93 expression and decreased miR-135a expression may be effective indexes for predicting prognosis in AMI patients complicated by pulmonary infection.