LGG预防油/脂致小鼠肥胖及调控肠道菌群作用

Prevention of Obesity Induced by Oil/Fat and Regulation of Intestinal Flora in mice by LGG

选用玉米油(油)与猪油(脂)探究不同油脂导致BALB/c小鼠肥胖的差异,分析鼠李糖乳杆菌(ATCC 53103,LGG)对肥胖的预防及肠道菌群的调控作用。50只5周龄BALB/c雄性小鼠被分为5组,普通饲料的正常组(CT)、猪油高脂饲料组(S)、玉米油高脂饲料组(P)、猪油高脂饲料+LGG灌胃组(SL)、玉米油高脂饲料+LGG灌胃组(PL)。喂养8周,进行指标检测。结果显示:猪油比玉米油更易引起小鼠肥胖及肠道菌群紊乱。S组小鼠体质量(41.42±2.30) g,体质量指数(BMI,4.24kg/m^(2)±0.26 kg/m^(2))、Lee’s指数(351.44±9.06)、脂肪系数(118.87±3.91) mg/g、血清总甘油三脂(TG,0.81 mmol/L±0.08 mmol/L)、低密度脂蛋白胆固醇(LDL-C,0.29 mmol/L±0.01 mmol/L)、丙氨酸氨基转移酶(ALT,53.42 U/L±0.95 U/L)及天门冬氨酸氨基转移酶(AST,164.65 U/L±9.35 U/L)均高于P组(P<0.05),而P组仅肝脏指数(51.07±1.35) mg/g高于S组(47.32 mg/g±1.93 mg/g,P<0.05)。小鼠肠道菌群差异菌种中,S组拟杆菌门(8.69±4.68)%和CF231(0.31±0.19)%相对丰度降低,别样棒菌属相对丰度(2.19±1.47)%升高,且F/B值(9.15±1.19)高于P组(4.25±0.57,P<0.05);而P组仅链球菌属丰度(14.41±6.37)%略高于S组(4.91±2.23)%。另一方面,LGG干预对猪油引起的小鼠肥胖的预防作用及肠道菌群紊乱的调控作用更为有效。SL组小鼠体质量(34.53±1.53) g、脂肪系数(26.10±2.51) mg/g均低于PL组(37.69 g±2.41 g,65.88 mg/g±1.98 mg/g,P<0.05),且SL组中LGG上调拟杆菌属的效果较为显著(P<0.05)。PL组中LGG虽下调厚壁菌门显著(P<0.05),但不能调控脱铁杆菌门相对丰度(P>0.05)。结论:猪油比玉米油更易引起肥胖,LGG对其肥胖的预防及肠道菌群的调控更为有效。

Lard and corn oil were used to explore the difference of obesity in BALB/c mice caused by oil and fat.And the effects of Lactobacillus rhamnosus ATCC 53103(LGG) on prevention of obesity and regulation of mice intestinal flora were analyzed.A total of 50 5-week-old BALB/c male mice were randomly divided into 5 groups included control check(CT),high-lard fed group(S),high-corn oil fed group(P),high-lard fed + LGG gavage group(SL) and high-corn oil fed + LGG gavage group(PL).The experiment lasted for 8 weeks and the related indexes were detected.The results showed that obesity was much easier to be caused by excessive lard compared with excessive corn oil.The body weight(41.42±2.30) g,body mass index(BMI,4.24 kg/m~2±0.26 kg/m~2),Lee's index(351.44±9.06),fat coefficient(118.87±3.91) mg/g,total triglyceride(TG,0.81 mmol/L±0.08 mmol/L),low density lipoprotein cholesterol(LDL-C,0.29mmol/L±0.01 mmol/L),alanine aminotransferase(ALT,53.42 U/L±0.95 U/L) and aspartate aminotransferase(AST,164.65U/L±9.35 U/L) were all significantly higher in mice in group S than in group P while only the liver index was higher in mice in group P(51.07±1.35) mg/g than in group S(47.32 mg/g±1.93 mg/g,P<0.05).Amongst the changed intestinal flora in mice,the relative abundance of Bacteroidetes(8.69±4.68)% and CF231(0.31±0.19)% decreased while Allobaculum(2.19±1.47)% increased in group S.And the F/B ratio was higher in group S(9.15±1.19) than that in group P(4.25±0.57,P<0.05).Comparatively,only the relative abundance of Streptococcus was slightly higher in mice in group P(14.41±6.37)% than in group S(4.91±2.23)%.However,LGG intervention was more effective in preventing obesity and regulating intestinal flora disorder in mice caused by lard.The body weight and fat coefficient of mice were lower in group SL(34.53 g±1.53 g,26.10 mg/g±2.51 mg/g) compared with in group PL(37.69 g±2.41 g,65.88 mg/g±1.98 mg/g,P<0.05).And the up-regulation of Bacteroides was more obviously in group SL.The down-regulation of Firmicutes was observed,but the regulation of Deferribacteres could not be conducted by LGG in group PL(P>0.05).In conclusion,obesity was more likely to be caused by lard than by corn oil.But LGG was more effectively in preventing obesity and regulating intestinal flora disorder caused by lard.