石榴皮活性物质提取及其W/O/W乳液稳态化包埋研究

Studies on the Extraction of Active Substances from Pomegranate Peel and Its Steady State Embedding of W/O/W Emulsion

石榴是药食同源的水果,在加工过程会产生大量石榴皮渣等副产物。为提高石榴副产物利用率,本文采用不同方法提取石榴皮活性成分,研究其抗氧化活性。采用水包油包水型乳液(W/O/W乳液)对其进行稳态化包埋,研究W/O/W对多酚稳定性及生物利用率的影响。结果表明:相比超高压提取和酶法提取,超声辅助醇提法具有较高的多酚提取率,为25%。石榴皮渣活性提取物对DPPH、羟基自由基清除力、总还原力好于VC;ABTS+清除活性与VC无显著差异;在质量浓度0.4mg/mL以下时,对超氧阴离子清除能力高于VC。W/O/W乳液能提高石榴皮渣活性提取物的储藏稳定性,且可使石榴皮活性物质免受体外胃消化的影响,靶向地在模拟小肠内传递和释放。本研究为石榴皮副产物的深加工提供了理论参考。

Pomegranate is a medicinal food,and a large amount of pomegranate peel and other by-products are produced during processing.In order to improve the utilization of pomegranate by-products,the active ingredients of pomegranate peel were extracted by different methods and their antioxidant activities were investigated in this paper.Subsequently,these bioactives were steadily encapsulated by water-in-oil-in-water emulsion(W/O/W) emulsion and the effect of W/O/W on the stability and bioavailability of polyphenols was investigated.The results showed that the ultrasound-assisted alcoholic extraction obtained higher yield of polyphenols with 25% than the ultra-high pressure extraction and enzymatic assisted extraction.The DPPH,hydroxyl radical scavenging and total reducing power of bioactives extracted from pomegranate peel was better than VC.While the ABTS+scavenging activity of the bioactives was not significantly different from that of VC.And its scavenging activity of superoxide anion was higher than that of VC when its concentration was lower than 0.4 mg/mL.W/O/W emulsion could improve the storage stability of the bioactive extracted from pomegranate peel,and protect the active substances in gastric digestion to targeted delivery and release in the simulated small intestine.This study provides theoretical reference for the further processing of pomegranate peel by-products.