酶@氢键有机框架复合材料的原位组装方法

In situ assembly of enzyme@hydrogen-bonded organic frameworks composites

利用纳米限域效应固定天然酶是设计新一代多功能、高稳定生物复合材料的重要策略.氢键有机框架(hydrogen-bonded organic frameworks,HOFs)是一类由有机分子通过氢键作用有序连接而成的多孔材料,具有结晶条件温和、骨架生物相容性高、化学组分丰富、孔隙率高和溶剂加工性优异等优点.开发生物相容的组装技术,调控HOF在酶表面的原位生长是一项前沿的纳米生物技术,该技术可合理整合HOF的框架化学特性和酶分子的生物学功能,在化学、生物和材料科学等交叉领域引起了广泛关注.本文介绍了原位组装酶@HOF复合材料的基本原理,总结了酶@HOF复合材料在生物传感、蛋白质递送、抗菌治疗、纳米马达和水体修复等的研究进展,最后讨论了这一领域面临的挑战和未来的发展方向.

Immobilization of natural enzymes using nanoconfinement effect is an efficient means to access a new generation of multifunctional and highly stable biocomposites.Hydrogen-bonded organic frameworks(HOFs),a class of porous materials formed by orderly connection of organic molecules via hydrogen bonding,feature with the merits of mild crystallization conditions,high backbone biocompatibility,rich chemical composition,high porosity and excellent solvent processability.The development of biocompatible assembly technology to regulate the in-situ growth of HOF on the surface of enzymes is a cutting-edge nanobiotechnology,which can reasonably integrate the framework chemistry of HOF and the biological function of enzyme molecules,gaining increasing attention in the interdisciplinary fields of chemistry,biological and materials science.In this review,we focus on the basic principles for the in situ assembly of enzyme@HOF biocomposites,and summarize the research progress of enzyme@HOF biocomposites in the domains of biosensing,protein delivery,antimicrobial therapy,nanomotors,and water remediation.Finally,we discussed the challenges facing this area and the way forward.