2021-2023年甘肃省肉与肉制品中沙门菌流行状况及病原学特征分析

Epidemiological status and etiological characteristics of Salmonella detected in meat and meat products in Gansu,2021−2023

目的 了解2021-2023年甘肃省肉与肉制品中沙门菌的流行情况及毒力基因分布,为甘肃省肉与肉制品中沙门菌的风险识别提供基础研究数据。方法 使用illumina二代测序平台对2021-2023年甘肃省农贸市场、批发市场、超市和零售加工店采集的样品分离的沙门菌进行全基因组测序,测序数据导入BioNumerics 7.6软件进行血清型分析、多位点序列分型分析、聚类分析和毒力基因筛查。结果 采集683份肉与肉制品样品,分离出的44株沙门菌分为16种血清型,其中肠炎沙门菌为优势血清型。共鉴定出17种序列型(ST),其中ST11为优势型。44株沙门菌分成3个不同进化分支,不同年份和不同地区菌株分散在不同进化分支中。共筛查出157种毒力基因,其中98种毒力基因在44株沙门菌中均存在,其他59种毒力基因呈不同比例分布在44株沙门菌中。结论 2021-2023年甘肃省肉与肉制品中的沙门菌以肠炎沙门菌(ST11)为主,血清型和ST型有一定的相关性,不同年份和地区菌株之间有一定的同源性。主要携带沙门菌毒力岛毒力基因和结构性毒力因子。

Objective To understand the etiological characteristics of Salmonella detected in meat and meat products in Gansu province from 2021 to 2023,and provide basic research data for risk identification of Salmonella in meat and meat products in Gansu.Methods Whole genome sequencing of strains of Salmonella isolated from meat and meat products collected from farm produce markets,wholesale markets,supermarkets,and retail processing stores in Gansu from 2021 to 2023 was performed by using illumina second-generation sequencing platform.The sequencing data were used for serotype analysis,multilocus sequence typing,clustering analysis and virulence gene screening with software BioNumerics 7.6.Results The 44 strains of Salmonella isolated from 683 meat and meat products collected,44 strains of Salmonella could be divided into 16 serotypes,of which Salmonella Enteritidis was predominant.A total of 17 sequence types(ST)were identified,and ST11 was predominant.The 44 strains of Salmonella were divided into 3 different clades,and the strains in different clades were detected in different years and different areas.A total of 157 virulence genes were detected,of which 98 virulence genes were detected in all the 44 strains of Salmonella,and the remaining 59 virulence genes were detected in 44 strains of Salmonella in different proportions.Conclusion Salmonella Enteritidis,belonging to ST11 and carrying Salmonella pathogenicity islands virulence genes and structural virulence factors,was mainly detected in meat and meat products in Gansu from 2021 to 2023,and the serotype was related to the sequence type to a certain extent,and the strains in different years and different areas shared homology to some degree.