红曲产品中Monacolin K含量检测方法的比较

Comparison of Detection Methods for Monacolin K content in Monascus Products

Monacolin K (MK)是红曲发酵产物中常见的成分。虽然开发各种定量技术检测红曲产品中的MK,但大多需要昂贵的设备和专门培训的人员。基于MK与杂质在固定相上吸附能力的不同以及MK在波长246 nm与254 nm吸光度差值ΔA,研究吸光度差值与MK浓度的关系。以80%乙醇为红曲发酵液中MK的提取剂,对比并探讨紫外单波长检测和样品采用层析处理前后紫外双波长检测对红曲样品中MK测定的准确性,结果发现样品经层析分离处理后使用紫外双波长检测法检测红曲产品中MK的方法准确性很高,相关系数0.925、回收率107.89%、精密度2.1%,方法最低检出限为29.5 mg/kg。在没有高效液相色谱的条件下可用于红曲样品中MK的检测分析。

Monacolin K(MK)is a common component in the fermentation products of Monascus.Although various quantitative techniques have been developed to detect MK in Monascus products,most require expensive equipment and specially trained personnel.Based on the different adsorption capacities of MK and impurities on fixed phases,as well as the differenceΔA in absorbance of MK at wavelengths of 246 nm and 254 nm,the relationship between absorbance difference and MK concentration was studied.Using 80%ethanol as the extractant for MK in Monascus fermentation broth,a comparative study was conducted on the accuracy of single wavelength UV detection and dual wavelength UV detection before and after chromatography for determining MK in red yeast samples.It was found that the method of using dual wavelength UV detection to detect MK in red yeast products after chromatography separation was highly accurate,with a correlation coefficient of 0.925,a recovery rate of 104.56%±1.43%,a precision of 1.4%,and a minimum detection limit of 1.52 mg/kg.This method can replace the expensive high-performance liquid chromatography method,and can be used for quality control of Monascus fermentation products under general experimental conditions.