透明质酸在促进肠道健康中的作用

Role of Hyaluronic Acid in Promoting Intestinal Health

目的研究透明质酸(hyaluronic acid,HA)对小鼠肠道健康的影响。方法利用DSS诱导的溃疡性结肠炎小鼠模型,测量小鼠结肠长度,进行DAI、CMDI评分和组织病理学检查,评估口服HA对小鼠溃疡性结肠炎的作用;建立小鼠小肠蠕动抑制模型和便秘模型,计算墨汁推进率、首粒排黑便时间、黑便重量和数量,评估口服HA对小鼠润肠通便功能的影响;通过细胞免疫功能、体液免疫功能、单核-巨噬细胞功能和NK细胞活性测定,评价口服HA对小鼠免疫功能的影响。结果HA对DSS诱导的小鼠溃疡性结肠炎的影响实验结果显示,HA低、中剂量组结肠长度显著升高,CMDI评分显著降低,组织学评分显著下降,表明HA能有效缓解DSS导致的小鼠结肠缩减、黏膜损伤等症状。小肠运动实验结果显示,HA各剂量组墨汁推进率均显著升高,表明HA可促进小肠蠕动;排便功能实验显示,HA各剂量组的首粒排黑便时间显著缩短,黑便重量和粒数显著增加,表明HA能促进小鼠排便。HA可提高细胞免疫功能、体液免疫功能及单核-巨噬细胞功能。结论HA具有改善肠道炎症、润肠通便和增强免疫力的作用。

Objective To explore the effect of hyaluronic acid(HA)on intestinal health in mice.Methods Using DSS induced ulcerative colitis mouse model,the colon length of mice was measured,and DAI and CMDI scoring and histopathological examination were performed to evaluate the effect of oral administration of HA on ulcerative colitis in mice.The small intestine peristalsis inhibition model and constipation model in mice were established,and the ink propulsion rate,the first black stool time,the weight and number of black stools were calculated to evaluate the effect of oral administration of HA on the laxative function of mice.The effects of oral administration of HA on the immune function of mice were evaluated by the determination of cellular immunity,humoral immunity,monocyte-macrophage function and NK cell activity.Results The experimental results of the effect of HA on DSS induced ulcerative colitis in mice showed that the colon lengths of mice in low and medium doses of HA groups were significantly increased,the CMDI scores were significantly decreased,and the histological scores were significantly decreased,indicating that HA can effectively alleviate the symptoms of DSS induced colon reduction and mucosal injury in mice.The results of small intestinal motility experiment showed that the ink propulsion rate of each HA dose group was significantly increased,indicating that HA could promote small intestinal peristalsis.The results of defecation function experiments showed that the first black stool time of each HA dose group was significantly shortened,and the weight and number of black stools were significantly increased,indicating that HA could promote defecation in mice.HA could also improve cellular immunity,humoral immunity and monocyte-macrophage function.Conclusion HA can improve intestinal inflammation,loosen bowel to relieve constipation,and enhance immunity.