甘蔗赤霉素合成基因ScGA3ox的功能初步研究

Preliminary Study on the Function of the Gibberellin Synthesizing Gene ScGA3ox in Sugarcane

近年来在拟南芥、水稻等模式植物中对赤霉素生物合成途径的研究日益完善,而重要糖料作物甘蔗中的赤霉素生物合成途径尚未明确,为了进一步验证ScGA3ox的功能,开展了甘蔗赤霉素合成基因ScGA3ox的功能初步研究。本文通过构建甘蔗GA3氧化酶基因ScGA3ox的植物过量表达载体,并将该基因通过农杆菌介导法转化到拟南芥中来快速验证其在植株生长中的功能。结果表明:本研究成功构建了ScGA3ox过量表达载体,并转化拟南芥获得过表达转基因T2株系。ScGA3ox过量表达对拟南芥的表型产生了影响:ScGA3ox转基因拟南芥株系与野生植株(WT)在株高上存在较为明显的差异,ScGA3ox-1和ScGA3ox-2株系分别比野生植株矮5.04和2.33cm。甘蔗GA3氧化酶基因ScGA3ox对植物生长有重要影响,但其具体功能研究有待深入开展。本研究结果将为进一步解析ScGA3ox基因调控甘蔗节间长度的分子机理提供了理论支撑。

In recent years,studies on the biosynthesis pathway of gibberellin in model plants such as Arabidopsis and rice have become increasingly perfect,while the biosynthesis pathway of gibberellin in sugarcane has not been clarified.In order to further verify the function of Sc GA3ox,a preliminary study on the function of Sc GA3ox in sugarcane was carried out.In this study,the plant overexpression vector of sugarcane GA3 oxidase gene Sc GA3ox was constructed,and the gene was transformed into Arabidopsis thaliana by Agrobacterium-mediated method to rapidly verify its function in plant growth.The results showed that,Sc GA3ox overexpression vector was successfully constructed,and Arabidopsis thaliana was transformed to obtain overexpression transgenic T2 strain.Overexpression of Sc GA3ox affected the phenotype of Arabidopsis thaliana.There was a significant difference in plant height between Sc GA3ox transgenic Arabidopsis thaliana and wild plants(WT).Sc GA3ox-1 and Sc GA3ox-2 strains were 5.04 cm and 2.33 cm shorter than wild plants,respectively.Sugarcane GA3 oxidase gene Sc GA3ox has an important effect on plant growth,but its specific function needs to be further studied.The results of this study will provide theoretical support for further analysis of the molecular mechanism of Sc GA3ox gene regulating sugarcane internode length.