摘要
流式光片显微成像系统(LS-FIS)能够对斑马鱼等微小模式生物进行高分辨率和高通量的三维成像,在发育生物学和药物筛选领域具有广泛的应用前景。为了研究斑马鱼体内的不同结构之间的相关性,在原单色荧光LS-FIS成像的基础上拓展了双色成像功能。首先采用荧光微球完成双通道分辨率的标定,并利用尺度不变特征变换(SIFT)和随机采样一致(RANSAC)算法完成了双通道图像的配准和三维图像重建。进一步对斑马鱼血管和红细胞同时进行成像,获得了受精后4天和受精后9天的斑马鱼幼鱼血管和红细胞分布的三维图像,并对血管红细胞分布情况做了分析与讨论。
The light-sheet flow imaging system(LS-FIS)enables high-resolution,high-throughput three-dimensional imaging of miniature model organisms like zebrafish.This capability presents potentially broad application prospects in the fields of developmental biology and drug screening.To study correlations between zebrafish structures,this study expanded on the original monochromatic LS-FIS to enable dual-color fluorescence imaging.To calibrate the resolution of the dual fluorescent channels,fluorescent microspheres were employed.Registration of dual-channel images and reconstruction of three-dimensional volumes were achieved using scale-invariant feature transform(SIFT)in combination with the random sample consensus(RANSAC)algorithm.Subsequently,this system was used to perform simultaneous imaging of zebrafish vasculatures and erythrocytes.Three-dimensional images were obtained and analyzed,revealing the distribution of vasculatures and erythrocytes in zebrafish larvae at 4 and 9 days post-fertilization.
作者
李雨蒙
张一凡
杨光
李辉
Li Yumeng;Zhang Yifan;Yang Guang;Li Hui(School of Biomedical Engineering(Suzhou),University of Science and Technology of China,Suzhou 215163,Jiangsu,China;Jiangsu Key Laboratory of Medical Optics,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,Jiangsu,China;Division of Nanobiomedicine,Suzhou Institute of Nano-Tech and Nano-Bionics,Chinese Academy of Sciences,Suzhou 215123,Jiangsu,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2024年第16期76-82,共7页
Laser & Optoelectronics Progress
基金
国家自然科学基金(62205368)。
关键词
流式光片显微成像
双色三维成像
图像配准
斑马鱼血管与红细胞结构
light-sheet flow imaging
dual-color three-dimensional imaging
image registration
zebrafish vasculatures and erythrocytes
