薄叶卷柏的化学成分研究

Study on chemical constituents of Selaginella delicatula(Desv.)Alston

为探究薄叶卷柏中的化学成分,该文采用HP20大孔树脂柱层析、正相硅胶柱层析、Sephadex LH-20葡聚糖凝胶柱层析以及半制备型液相色谱等对薄叶卷柏乙酸乙酯部位进行分离纯化,并通过现代波谱学方法(NMR、MS、UV、IR)鉴定了18个由薄叶卷柏乙酸乙酯部位分离得到的化合物,分别鉴定为7-去甲基银杏双黄酮(1)、delicaflavone(2)、sequoiavone(3)、2,3,2″,3″-四氢扁柏双黄酮(4)、2,3,2″,3″-四氢穗花杉双黄酮(5)、2 S,2″S-四氢罗伯斯塔黄酮(6)、(2″S)-2″,3″-dihydrodelicaflavone(7)、2,3-二氢穗花杉双黄酮(8)、(2 S)-2,3-dihydrorobustaflavone-4′-methyl ether(9)、hinokiflavone(10)、穗花杉双黄酮(11)、(2″S)-2″,3″-dihydrorobustaflavone(12)、2,3-dihydro-robustaflavone(13)、2″,3″-二氢异柳杉双黄酮(14)、4′-甲氧基-罗伯斯塔黄酮(15)、蛇床子素(16)、6-(5-acetyl-2-methoxyphenyl)-apigenin(17)、芹菜素(18).其中16个化合物,化合物1~10、12~14、16~18为首次从薄叶卷柏中分离,化合物16为首次从卷柏属植物中分离得到.化合物10和化合物13与分离得到的其他化合物相比较能较好抑制食管癌Eca-109细胞的生长.

In order to study the chemical constituents of Selaginella delicatula(Desv.)Alston,HP20 macroporous resin column chromatography(CC),normal-phase silica gel CC,Sephadex LH-20 gel CC,and semi-preparative liquid chromatography were used to separate and purify the ethyl acetate fraction of S.delicatula.Eighteen compounds isolated from the ethyl acetate fraction of S.delicatula were identified by modern spectroscopic methods(NMR,MS,UV,IR).They were identified as bilobetin(1),delicaflavone(2),7-O-methyl-amentoflavone(3),2,3,2″,3″-tetrahydrohinokiflavone(4),2,3,2″,3″-tetrahydroamentoflavone(5),2 S,2″S-tetrahydrorobustaflavone(6),(2″S)-2″,3″-dihydrodelicaflavone(7),2,3-dihydroamentoflavone(8),(2 S)-2,3-dihydrorobustaflavone-4′-methyl ether(9),hinokiflavone(10),amentoflavone(11),(2″S)-2″,3″-dihydrorobustaflavone(12),2,3-dihydro-robustaflavone(13),2″,3″-dihydroisocryptomerin(14),robustaflavone-4′-methyl ether(15),osthol(16),6-(5-acetyl-2-methoxyphenyl)-apigenin(17),apigenin(18),respectively.Among them,16 compounds including 1-10,12-14,and 16-18 were firstly isolated from this title plant,and compound 16 was firstly isolated from the Selaginella genus.Compound 10 and compound 13 were able to inhibit the growth of the Eca-109 cell line to a certain extent at a concentration of 55.35μmol·L^(-1) and 75.89μmol·L^(-1),respectively.